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PXD016017

PXD016017 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA Pluripotent Stem Cell-Derived Matrix with Potent Osteoregenerative Capabilities
DescriptionApproximately 10% of fractures that occur in the United States annually will not heal without intervention. This figure is rising due to an aging population and a rise in conditions that impair bone healing. Current treatments can be marginally effective, costly, and some have adverse effects. A safe and manufacturable material that mimics anabolic bone is the holy grail of bone tissue engineering, but achieving this is challenging. Adult stem cells, especially mesenchymal stem cells (MSCs), are excellent candidates for engineering bone, but the lack of reproducibility due to donor variation and culture protocols have hindered progress in this area. Herein, we describe an MSC line generated from induced pluripotent stem cells with remarkable osteogenic properties. The cells can be induced to generate large amounts of osteogenic extracellular matrix (ihMatrix) under monolayer culture conditions. When pure ihMatrix is implanted into calvarial defects in mice, it has intrinsic osteogenic activity that significantly surpasses the efficacy of bone morphogenic protein 2 resulting in complete healing of defects in 4 weeks through a mechanism of action mediated in part by collagen VI and XII. Based on these findings, we propose that ihMatrix could represent a superior replacement for the current gold standards, autograft and BMP products, used commonly in bone tissue engineering.
HostingRepositoryPRIDE
AnnounceDate2020-07-02
AnnouncementXMLSubmission_2020-07-01_22:28:07.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD016017
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterCarl Gregory
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListOxidation; Carbamidomethyl
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-10-25 02:34:38ID requested
12020-07-01 22:28:08announced
Publication List
McNeill EP, Zeitouni S, Pan S, Haskell A, Cesarek M, Tahan D, Clough BH, Krause U, Dobson LK, Garcia M, Kung C, Zhao Q, Saunders WB, Liu F, Kaunas R, Gregory CA, Characterization of a pluripotent stem cell-derived matrix with powerful osteoregenerative capabilities. Nat Commun, 11(1):3025(2020) [pubmed]
Keyword List
submitter keyword: Stem Cell´╝î Matrix
Contact List
Carl A. Gregory
contact affiliationDepartment of Molecular and Cellular Medicine, Institute for Regenerative Medicine, Texas A&M Health Science Center, College Station, TX, 77843, USA.
contact emailcgregory@tamu.edu
lab head
Carl Gregory
contact affiliationTexas A&M Health Science Center
contact emailcgregory@tamu.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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