PXD015976-2

PXD015976 is an original dataset announced via ProteomeXchange.

Title	Low dose perfluorooctanesulfonic acid (PFOS) and perfluorohexanesulfonic acid (PFHxS) augment hepatic steatosis and shift the sera lipidome in a high fat high carbohydrate diet.
Description	Non-alcoholic fatty liver disease (NAFLD) is most prevalent form of liver disease, affecting over 30% of Americans. Perfluoroalkyl substances (PFAS) represent a family of environmental toxicants that have infiltrated the living world. This study explores diet-PFAS interactions and their potential role in the increasing global incidence of NAFLD. Male C57BL/6 mice were fed with either a low-fat diet (11% kcal from fat) or a high fat (58% kcal from fat) high carbohydrate (42g/L) diet with or without PFOS or PFHxS in feed (0.0003% w/w) for 29 weeks. Proteomic, lipidomic, and gene expression measurement techniques were utilized to explore mechanistic pathways. With administration of a high fat high carbohydrate (HFHC) diet, PFOS and PFHxS augmented macrovesicular steatosis, indicative of fatty liver. There was a clear shift in the lipidome of the serum phosphatidylcholines, phosphatidylethanolamines, and triglycerides with PFAS exposure. Finally, chain length exerted significant influence on tissue partitioning and the resulting hepatic gene and protein signatures of PFHxS and PFOS in vivo.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:12:15.163.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marisa Pfohl
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-23 01:28:56	ID requested
1	2020-09-14 22:38:42	announced
⏵ 2	2024-10-22 05:12:15	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: PFOS, High Fat Diet, Hepatotoxicity,Fatty Liver Disease, PFHxS, NAFLD

Angela Slitt
contact affiliation	Biomedical and Pharmaceutical Sciences, University of Rhode Island, Kingston, RI, 02881, USA
contact email	angela_slitt@uri.edu
lab head
Marisa Pfohl
contact affiliation	University of Rhode Island
contact email	marisa_pfohl@uri.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015976
     1. Label: PRIDE project
     2. Name: Low dose perfluorooctanesulfonic acid (PFOS) and perfluorohexanesulfonic acid (PFHxS) augment hepatic steatosis and shift the sera lipidome in a high fat high carbohydrate diet.