<<< Full experiment listing


PXD015976 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLow dose perfluorooctanesulfonic acid (PFOS) and perfluorohexanesulfonic acid (PFHxS) augment hepatic steatosis and shift the sera lipidome in a high fat high carbohydrate diet.
DescriptionNon-alcoholic fatty liver disease (NAFLD) is most prevalent form of liver disease, affecting over 30% of Americans. Perfluoroalkyl substances (PFAS) represent a family of environmental toxicants that have infiltrated the living world. This study explores diet-PFAS interactions and their potential role in the increasing global incidence of NAFLD. Male C57BL/6 mice were fed with either a low-fat diet (11% kcal from fat) or a high fat (58% kcal from fat) high carbohydrate (42g/L) diet with or without PFOS or PFHxS in feed (0.0003% w/w) for 29 weeks. Proteomic, lipidomic, and gene expression measurement techniques were utilized to explore mechanistic pathways. With administration of a high fat high carbohydrate (HFHC) diet, PFOS and PFHxS augmented macrovesicular steatosis, indicative of fatty liver. There was a clear shift in the lipidome of the serum phosphatidylcholines, phosphatidylethanolamines, and triglycerides with PFAS exposure. Finally, chain length exerted significant influence on tissue partitioning and the resulting hepatic gene and protein signatures of PFHxS and PFOS in vivo.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMarisa Pfohl
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListiodoacetamide derivatized residue
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-10-23 01:28:56ID requested
12020-09-14 22:38:42announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Fatty Liver Disease, PFHxS, PFOS, NAFLD, High Fat Diet, Hepatotoxicity
Contact List
Angela Slitt
contact affiliationBiomedical and Pharmaceutical Sciences, University of Rhode Island, Kingston, RI, 02881, USA
contact emailangela_slitt@uri.edu
lab head
Marisa Pfohl
contact affiliationUniversity of Rhode Island
contact emailmarisa_pfohl@uri.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD015976
PRIDE project URI
Repository Record List
[ + ]