PXD015963-1
PXD015963 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comparative phosphorylation map of Dishevelled 3 links phospho-signatures to biological outputs. |
Description | Background: Dishevelled (DVL) is an essential component of the Wnt signaling cascades. Function of DVL is controlled by phosphorylation but the molecular details are missing. DVL3 contains 131 serines and threonines whose phosphorylation generates complex barcodes underlying diverse DVL3 functions. In order to dissect the role of DVL phosphorylation we analyzed the phosphorylation of human DVL3 induced by previously reported (CK1ε, NEK2, PLK1, CK2α, RIPK4, PKCδ) and newly identified (TTBK2, Aurora A) DVL kinases. Methods: Shotgun proteomics including TiO2 enrichment of phosphorylated peptides followed by liquid chromatography tandem mass spectrometry on immunoprecipitates from HEK293T cells was used to identify and quantify phosphorylation of DVL3 protein induced by 8 kinases. Functional characterization was performed by in-cell analysis of phospho-mimicking/non phosphorylatable DVL3 mutants and supported by FRET assays and NMR spectroscopy. Results: We used quantitative mass spectrometry and calculated site occupancies and quantified phosphorylation of >80 residues. Functional validation demonstrated the importance of CK1ε-induced phosphorylation of S268 and S311 for Wnt-3a-induced β-catenin activation. S630-643 cluster phosphorylation by CK1, NEK2 or TTBK2 is essential for even subcellular distribution of DVL3 when induced by CK1 and TTBK2 but not by NEK2. Further investigation showed that NEK2 utilizes a different mechanism to promote even localization of DVL3. NEK2 triggered phosphorylation of PDZ domain at S263 and S280 prevents binding of DVL terminus to PDZ and promotes an open conformation of DVL3 that is more prone to even subcellular localization. Conclusions: We identify unique phosphorylation barcodes associated with DVL function. Our data provide an example of functional synergy between phosphorylation in structured domains and unstructured IDRs that together dictate the biological outcome. |
HostingRepository | PRIDE |
AnnounceDate | 2020-01-09 |
AnnouncementXML | Submission_2020-01-09_04:32:39.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Katerina Hanáková |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2019-10-22 06:31:34 | ID requested | |
⏵ 1 | 2020-01-09 04:32:40 | announced | |
2 | 2024-10-22 03:59:04 | announced | 2024-10-22: Updated project metadata. |
Publication List
Han, á, kov, á K, Bernat, í, k O, Kravec M, Micka M, Kumar J, Harno, š J, Ovesn, á P, Pacl, í, kov, á P, R, á, dsetoulal M, Pot, ě, š, il D, Tripsianes K, Č, aj, á, nek L, Zdr, á, hal Z, Bryja V, Comparative phosphorylation map of Dishevelled 3 links phospho-signatures to biological outputs. Cell Commun Signal, 17(1):170(2019) [pubmed] |
Keyword List
submitter keyword: Dishevelled/DVL3/phosphorylation/kinase/mass spectrometry/CK1/TTBK2/NEK2/Wnt |
Contact List
Zbynek Zdrahal | |
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contact affiliation | CEITEC—Central European Institute of Technology, Masaryk University, Brno, Czech Republic National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Brno, Czech Republic |
contact email | zdrahal@sci.muni.cz |
lab head | |
Katerina Hanáková | |
contact affiliation | CEITEC MU |
contact email | katerina.hanak@gmail.com |
dataset submitter |
Full Dataset Link List
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PRIDE project URI |
Repository Record List
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