PXD015931-2

PXD015931 is an original dataset announced via ProteomeXchange.

Title	Comparative quantitative proteomic analysis of HCMV infected cells highlights pUL138 as a multiple functional protein
Description	Human cytomegalovirus (HCMV) is a widespread virus and can establish life-long latent infection in large populations. In order to establish persistent and latent infection in healthy individuals, HCMV encodes a large array of proteins that can modulate different components and pathways of host cells. It has been reported that pUL138 encoded by UL133-UL138 polycistronic locus promotes a latent infection in primary CD34+ HPCs infected in vitro. In this study, a recombinant HCMV, namely HanUL138del, was constructed by deleting the UL138 locus of Han, a clinic HCMV strain. Then a comparative quantitative proteomic analysis of Han and HanUL138del infected MRC5 cells was performed, aiming to study the effect of pUL138 on host cell in the context of HCMV infection. Our result indicated that at the early phase of HCMV infection, innate immune response was differentially activated, while at late phase of HCMV infection, multiple host proteins were differentially expressed, between Han or HanUL138del infected cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:26:42.990.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Leike Zhang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-21 01:44:59	ID requested
1	2021-09-08 14:09:41	announced
⏵ 2	2024-10-22 05:26:43	announced	2024-10-22: Updated project metadata.

Li Y, Shang W, Xiao G, Zhang LK, Zheng C, A Comparative Quantitative Proteomic Analysis of HCMV-Infected Cells Highlights pUL138 as a Multifunctional Protein. Molecules, 25(11):(2020) [pubmed]

10.3390/molecules25112520;

submitter keyword: UL138,HCMV, quantitative proteomics, persistent infection

Leike Zhang
contact affiliation	State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, P. R. China
contact email	kebike1986@126.com
lab head
Leike Zhang
contact affiliation	IOV
contact email	kebike1986@126.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD015931

PRIDE project URI

• PRIDE
  1. PXD015931
     1. Label: PRIDE project
     2. Name: Comparative quantitative proteomic analysis of HCMV infected cells highlights pUL138 as a multiple functional protein