PXD015928-2

PXD015928 is an original dataset announced via ProteomeXchange.

Title	Heterogeneity of proteome dynamics between connective tissue phases of adult tendon
Description	Turnover of matrix proteins is essential to maintain tissue homeostasis, and dysregulation of protein turnover has been implicated in a variety of diseases. However, proteome dynamics in the musculoskeletal system, particularly tendon, remain relatively unexplored. The aim of this study was therefore to calculate the turnover rate of individual proteins within tendon and its constituent components. We hypothesised that turnover of proteins in the interfascicular matrix would be greater than in the fascicles. Achilles tendons were harvested from rats fed heavy labelled water over a period of 127 days. Proteins were extracted from one Achilles tendon, while laser capture microdissection was used to isolate regions of interfascicular and fascicular matrix from the other Achilles prior to protein extraction. Samples were analysed using tandem mass spectrometry and the rate of label incorporation used to calculate turnover rate of individual proteins. Results demonstrate complex proteome dynamics within tendon, with differences in protein turnover rates approaching 1000-fold. Collagen turnover was relatively slow, with much more rapid turnover of proteoglycans and glycoproteins. Data support the hypothesis, demonstrating overall faster protein turnover within the interfascicular matrix compared to the fascicles. More rapid turnover of the interfascicular matrix may be a mechanism to repair microdamage to this region which is exposed to high levels of shear during locomotion. The techniques used here provide a powerful approach to interrogate alterations in protein turnover in the musculoskeletal system with ageing and/or disease which are likely to influence injury risk.
HostingRepository	PRIDE
AnnounceDate	2020-05-04
AnnouncementXML	Submission_2020-05-17_23:49:15.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD015928
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Chavaunne Thorpe
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-21 01:44:23	ID requested
1	2020-05-04 09:47:10	announced
⏵ 2	2020-05-17 23:49:16	announced	2020-05-18: Updated publication reference for PubMed record(s): 32393437.

Choi H, Simpson D, Wang D, Prescott M, Pitsillides AA, Dudhia J, Clegg PD, Ping P, Thorpe CT, Heterogeneity of proteome dynamics between connective tissue phases of adult tendon. Elife, 9():(2020) [pubmed]

submitter keyword: Tendon, rat, laser capture, isotope labelling, LC-MS/MS

Chavaunne Thorpe
contact affiliation	Comparative Biomedical Sciences Royal Veterinary College Royal College Street London, NW1 0TU
contact email	cthorpe@rvc.ac.uk
lab head
Chavaunne Thorpe
contact affiliation	Royal Veterinary College
contact email	cthorpe@rvc.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015928
     1. Label: PRIDE project
     2. Name: Heterogeneity of proteome dynamics between connective tissue phases of adult tendon