PXD015919-2
PXD015919 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Membrane protein landscape of plant nuclear envelope and nuclear pore complex |
| Description | We used biochemical fractionation coupled with the subtractive proteomics to profile PNET proteins. Both the crude NE and the total MM fraction were subject to label-free quantitative mass spectrometry (LFQMS). We also adapted proximity labeling-coupled LFQMS (PL-LFQMS) method to map the PNET proteome. Overall, We identified over 200 potential candidates for plant nuclear envelope transmembrane (PNET) proteins and confirmed 14 PNETs. |
| HostingRepository | iProX |
| AnnounceDate | 2020-03-11 |
| AnnouncementXML | Submission_2020-11-19_00:29:17.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yu Tang |
| SpeciesList | scientific name: Arabidopsis thaliana; NCBI TaxID: 3702; |
| ModificationList | N6-biotinyl-L-lysine |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2019-10-20 03:11:04 | ID requested | |
| 1 | 2020-03-10 21:25:49 | announced | |
| ⏵ 2 | 2020-11-19 00:29:18 | announced | 2020-11-19: Update publication information |
Publication List
| Tang Y, Huang A, Gu Y, Global profiling of plant nuclear membrane proteome in Arabidopsis. Nat Plants, 6(7):838-847(2020) [pubmed] |
Keyword List
| submitter keyword: Arabidopsis seedling, PNET, NE, MM, BioID2, LFQMS, PL-LFQMS |
Contact List
| Yangnan Gu | |
|---|---|
| contact affiliation | Department of Plant & Microbial Biology, University of California, Berkeley |
| contact email | guyangnan@berkeley.edu |
| lab head | |
| Yu Tang | |
| contact affiliation | Tsinghua University |
| contact email | tang414yu@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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