PXD015917-2

PXD015917 is an original dataset announced via ProteomeXchange.

Title	5-fluorouracil (5-Fu) resistant Bel/5Fu cell line and its parent Bel7402 cell line
Description	The extensive drug resistance of hepatocellular carcinoma (HCC) has become the main cause of chemotherapy failure. The mechanisms leading to drug resistance in tumor cells are not well understood. Previous studies demonstrated that plasma membrane proteins play important roles in mediating drug disposal or pharmacokinetics. Therefore, we performed proteomic studies on the composition of 5-fluorouracil (5-Fu) resistant Bel/5Fu cell line and its parent Bel7402 cell line by using an ionic liquid-assisted proteins extraction method with the advantage of extracting plasma membrane proteins to a greater extent. In total, 4,532 unique proteins were quantified and 103 proteins (>2-fold change, p-value <0.05) including 25 plasma membrane proteins showed to be differentially expressed in Bel/5Fu compared with Bel7402. Western blot analysis results were further confirmed that the plasma membrane proteins EPHX1 PLIN2 RAB27B SLC4A2 were up-regulated in Bel/5Fu cells in accordance with the proteomics data. Moreover, cell viability assay and clonogenic survival assay results demonstrated that EPHX1 are closely related to the chemoresistance of Bel/5Fu to 5Fu.
HostingRepository	iProX
AnnounceDate	2019-10-19
AnnouncementXML	Submission_2020-11-19_00:26:52.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sun Rui
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-18 23:16:24	ID requested
1	2019-10-18 23:19:50	announced
⏵ 2	2020-11-19 00:26:53	announced	2020-11-19: Update publication information

Sun R, Dong C, Li R, Chu H, Liu J, Hao D, Zhang L, Zhao B, Wang L, Zhang Y, Proteomic Analysis Reveals that EPHX1 Contributes to 5-Fluorouracil Resistance in a Human Hepatocellular Carcinoma Cell Line. Proteomics Clin Appl, 14(4):e1900080(2020) [pubmed]

submitter keyword: hepatocellular carcinoma, ionic liquid, chemoresistance, plasma membrane protein

Wang Liming
contact affiliation	Division of Hepatobiliary and Pancreatic Surgery, Department of General Surgery, The Second Affiated Hospital of Dalian Medical University
contact email	wangbcc259@163.com
lab head
Sun Rui
contact affiliation	The Second Affiated Hospital of Dalian Medical University
contact email	sunrui199219@163.com
dataset submitter

iProX dataset URI