PXD015907 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Site-specific mapping and quantification of protein S-sulphenylation in cells |
Description | Jing Yang, Vinayak Gupta, Kate S. Carroll, & Daniel C. Liebler, (2014) Nature Communications 5, 4776, doi:10.1038/ncomms5776 Cysteine S-sulphenylation provides redox regulation of protein functions, but the global cellular impact of this transient post-translational modification remains unexplored. We describe a chemoproteomic workflow to map and quantify over 1,000 S-sulphenylation sites on more than 700 proteins in intact cells. Quantitative analysis of human cells stimulated with hydrogen peroxide or epidermal growth factor measured hundreds of site selective redox changes. Different cysteines in the same proteins displayed dramatic differences in susceptibility to S-sulphenylation. Newly discovered S-sulphenylations provided mechanistic support for proposed cysteine redox reactions and suggested novel redox mechanisms, including S-sulphenyl-mediated redox regulation of the transcription factor HIF1A by SIRT6. S-sulphenylation is favored at solvent-exposed protein surfaces and is associated with sequence motifs that are distinct from those for other thiol modifications. S-sulphenylations affect regulators of phosphorylation, acetylation and ubiquitylation, which suggest regulatory crosstalk between redox control and signalling pathways. |
HostingRepository | MassIVE |
AnnounceDate | 2024-04-09 |
AnnouncementXML | Submission_2024-04-09_10:10:50.407.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Xu Zhang |
SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
ModificationList | Carbamidomethyl; Oxidation; unknown modification; unknown modification |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-10-18 08:59:36 | ID requested | |
⏵ 1 | 2024-04-09 10:10:51 | announced | |
Publication List
Yang J, Gupta V, Carroll KS, Liebler DC, Site-specific mapping and quantification of protein S-sulphenylation in cells. Nat Commun, 5():4776(2014) [pubmed] |
Keyword List
Contact List
Daniel C Liebler |
contact affiliation | Vanderbilt University |
contact email | daniel.liebler@Vanderbilt.Edu |
lab head | |
Xu Zhang |
contact affiliation | NIH/NCI |
contact email | xu.zhang@nih.gov |
dataset submitter | |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location
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