PXD015876-1
PXD015876 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Mechanism for utilization of the plant-derived metabolite salicin by a bacterial co-culture |
| Description | Pseudomonas sp. GM16 associates with Populus, a model plant in biofuel production. Populus releases abundant phenolic glycosides such as salicin, but Pseudomonas sp. GM16 cannot utilize salicin whereas Pseudomonas strains are known to utilize compounds similar to the aglycone moiety of salicin – salicyl alcohol. We propose that the association of Pseudomonas to Populus is mediated by another organism (such as Rahnella sp. OV744) that degrades the glucosyl group of salicin. In this study, we demonstrate that in the Rahnella-Pseudomonas salicin co-culture model, Rahnella grows by degrading salicin to glucose 6-phosphate and salicyl alcohol which is secreted out and is subsequently utilized by Pseudomonas for its growth. Using various quantitative approaches, we elucidate the individual pathways for salicin and salicyl alcohol metabolism present in Rahnella and Pseudomonas, respectively. Furthermore, we were able to establish that the salicyl alcohol cross-feeding interaction between the two strains on salicin medium is carried out through combination of their respective individual pathways. The research presents one of the potential advantages of salicyl alcohol release by strains such as Rahnella, and how phenolic glycosides could be involved in attracting multiple types of bacteria into the Populus microbiome. |
| HostingRepository | MassIVE |
| AnnounceDate | 2023-01-11 |
| AnnouncementXML | Submission_2023-01-11_13:19:29.926.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Greg Hurst |
| SpeciesList | scientific name: Pseudomonas sp. GM16 and Rahnella sp. OV744; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | LTQ XL |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2019-10-17 13:10:05 | ID requested | |
| ⏵ 1 | 2023-01-11 13:19:30 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: co-culture, Populus microbiome, salicin metabolism |
Contact List
| Dale Pelletier | |
|---|---|
| contact affiliation | Oak Ridge National Laboratory |
| contact email | pelletierda@ornl.gov |
| lab head | |
| Greg Hurst | |
| contact affiliation | Oak Ridge National Laboratory |
| contact email | hurstgb@ornl.gov |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000084467/ |
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