PXD015874-1

PXD015874 is an original dataset announced via ProteomeXchange.

Title	SBDS-dependent ribosome destabilization maintains pluripotency via regulation of translation
Description	Unique traits of pluripotent stem cells are not fully known. Using thermal proteome profiling, we identified reduced stability of ribosomes in induced pluripotent cells (iPSCs) compared to that in somatic cells. Also, iPSCs exhibited lower protein synthesis rate and lower expression of a ribosome maturation factor, the Shwachman-Bodian-Diamond Syndrome protein (SBDS). Differentiation of iPSCs led to upregulation of SBDS and knock-down of SBDS slowed the differentiation while increasing expression of master pluripotency markers NANOG and OCT4. Mutations in the SBDS gene have been shown to impair ribosome assembly and to inhibit differentiation of hematopoietic stem cells causing the Shwachman-Diamond syndrome. Physiological SBDS-dependent destabilization of ribosomes appears to be a tool for translational control providing robustness to the state of pluripotency.
HostingRepository	PRIDE
AnnounceDate	2021-10-05
AnnouncementXML	Submission_2021-10-05_02:39:02.395.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pierre Sabatier
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-17 06:14:02	ID requested
⏵ 1	2021-10-05 02:39:02	announced
2	2024-10-22 05:29:40	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Pluripotent stem cells, ribosome, thermal proteome profiling, translation

Roman A. Zubarev
contact affiliation	Chemistry I, Department of Medical Biochemistry and Biophysics, Karolinska Institutet SE 17177 Stockholm, Sweden. Department of Pharmacological & Technological Chemistry, I.M. Sechenov First Moscow State Medical University, Moscow, 119146, Russia.
contact email	roman.zubarev@ki.se
lab head
Pierre Sabatier
contact affiliation	Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
contact email	pierre.sabatier@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/10/PXD015874

PRIDE project URI

• PRIDE
  1. PXD015874
     1. Label: PRIDE project
     2. Name: SBDS-dependent ribosome destabilization maintains pluripotency via regulation of translation