PXD015856-1

PXD015856 is an original dataset announced via ProteomeXchange.

Title	Protein partners identification in Pyrococcus abyssi by nanoLC-MSMS
Description	Recombinant aRNase J from P. abyssi, with C-terminal (His)6-tag (aRNase J-His), was produced, purified and used as baits for AP-MS experiments. In order to discriminate specific protein interaction from column background, we performed mock AP-MS analyses with P. abyssi cell extracts in absence of protein bait. In addition, to determine if some interactions are mediated through RNA or DNA, we implemented a nuclease treatment after incubating the cell extract with the bait. The co-purified partners were identified by bottom-up proteomic techniques coupled with mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2020-01-21
AnnouncementXML	Submission_2020-01-21_01:17:50.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lydie Correia
SpeciesList	scientific name: Pyrococcus abyssi; NCBI TaxID: 29292;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-16 05:46:15	ID requested
⏵ 1	2020-01-21 01:17:51	announced

Dataset with its publication pending

submitter keyword: Pyrococcus abyssi, pull-down, nanoLC-MSMS

Lydie Oliveira Correia
contact affiliation	INRA MICALIS PAPPSO
contact email	lydie.oliveira-correia@inra.fr
lab head
Lydie Correia
contact affiliation	INRA PAPPSO
contact email	lydie.oliveira-correia@inra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD015856

PRIDE project URI

• PRIDE
  1. PXD015856
     1. Label: PRIDE project
     2. Name: Protein partners identification in Pyrococcus abyssi by nanoLC-MSMS