PXD015854-1

PXD015854 is an original dataset announced via ProteomeXchange.

Title	Regulatory Role of S100A6 in Hematopoetic Stem Cells
Description	The fate options of hematopoietic stem cells (HSCs) include self-renewal, differentiation, migration and apoptosis, but the interaction between intracellular Ca2+ and cytoplasmic chaperon protein in regulating fate options of long term-HSCs (LT-HSC) is unknown. We created a S100A6 conditional knockout mouse model in the hematopoietic system and our studies showed that in S100A6KO, the number of LT-HSCs was significantly reduced and HSCs engrafted poorly. After 5FU challenge, the frequency of S100A6KO HSCs remained significantly low. Our data showed that S100A6 failed to self-renew through Akt pathway in an intracellular calcium (Cai2+)-dependent manner. Expression profiling of S100A6KO obtained from gene signatures revealed that cytosolic calcium level and proteins translocation to mitochondria were decreased. Mitochondrial oxidative phosphorylation was impaired in S100A6KO. Proteomic data indicated Hsp90 protein and chaperonin family were reduced. Our findings demonstrated that S100A6 regulates fate options of HSCs self-renewal through integrating Akt signaling, specifically governing mitochondria metabolic function and protein quality.
HostingRepository	PRIDE
AnnounceDate	2020-06-22
AnnouncementXML	Submission_2020-06-21_22:19:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Akos Vegvari
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-16 04:08:16	ID requested
⏵ 1	2020-06-21 22:19:09	announced
2	2024-10-22 05:07:40	announced	2024-10-22: Updated project metadata.

Grahn THM, Niroula A, V, é, gv, á, ri Á, Oburoglu L, Pertesi M, Warsi S, Safi F, Miharada N, Garcia SC, Siva K, Liu Y, R, ö, rby E, Nilsson B, Zubarev RA, Karlsson S, S100A6 is a critical regulator of hematopoietic stem cells. Leukemia, 34(12):3323-3337(2020) [pubmed]

submitter keyword: Mouse, hematopoetic stem cell, LC-MS/MS

Akos Vegvari
contact affiliation	Proteomics Biomedicum Core Facility, Div. of Chemistry I, Dept. Biochemistry & Biophysics, Karolinska Institutet, Stockholm, Sweden
contact email	akos.vegvari@ki.se
lab head
Akos Vegvari
contact affiliation	Karolinska Institutet
contact email	akos.vegvari@ki.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015854
     1. Label: PRIDE project
     2. Name: Regulatory Role of S100A6 in Hematopoetic Stem Cells