PXD015845-1

PXD015845 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomic Characterization of Signaling Networks Resulting from Activation of Chemokine Receptor CCR2
Description	Leukocyte recruitment, a universal feature of tissue inflammation, is regulated by the interactions of chemokines with their G protein-coupled receptors. Activation of CC chemokine receptor 2 (CCR2) by its cognate chemokine ligands, including CCL2, plays a central role in recruitment of monocytes in several inflammatory diseases. In this study, we used phosphoproteomics to enable unbiased characterization of the signaling network resulting from CCL2 activation of CCR2. Using data-independent acquisition (DIA) mass spectrometry, both the proteome and phosphoproteome were quantified for FlpIn-HEK293T cells stably expressing CCR2, at six time points after activation with CCL2, in comparison with untreated cells. Differential expression analysis identified 699 significantly regulated phosphorylation sites located on 441 proteins. As expected, many of these proteins participate in canonical signal transduction pathways and the regulation of actin cytoskeleton dynamics, including numerous guanine nucleotide exchange factors (GEFs) and GTPase activating proteins (GAPs). In addition, we identified regulated phosphorylation sites in numerous proteins that exert functions in the nucleus, including several constituents of the nuclear pore complex. This study provides an unprecedented level of detail about CCR2 signalling and identifies potential novel targets for regulation of CCR2 function.
HostingRepository	PRIDE
AnnounceDate	2020-04-08
AnnouncementXML	Submission_2020-04-07_23:11:41.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cheng Huang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-16 02:34:49	ID requested
⏵ 1	2020-04-07 23:11:42	announced
2	2024-10-22 05:01:53	announced	2024-10-22: Updated project metadata.

Huang C, Foster SR, Shah AD, Kleifeld O, Canals M, Schittenhelm RB, Stone MJ, Phosphoproteomic characterization of the signaling network resulting from activation of the chemokine receptor CCR2. J Biol Chem, 295(19):6518-6531(2020) [pubmed]

submitter keyword: Phosphoproteomics, DIA, CCR2, Signalling

Martin J Stone
contact affiliation	1Infection and Immunity Program and Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton 3800, Victoria, Australia
contact email	martin.stone@monash.edu
lab head
Cheng Huang
contact affiliation	Monash University
contact email	cheng.huang@monash.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015845
     1. Label: PRIDE project
     2. Name: Phosphoproteomic Characterization of Signaling Networks Resulting from Activation of Chemokine Receptor CCR2