PXD015838-1
PXD015838 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Targeted assay for differentiation and monitoring the concentration of GDF 8 (Myostatin) and GDF11 in mouse serum |
Description | Abstract Growth differentiation factor 11 (GDF11) and Myostatin (GDF8) are circulating factor shown to reduce cardiac hypertrophy of aged animals. Although GDF11 and GDF8 have high structural homology, it seems that GDF11 is had a more potent effect on reducing cardiomyocytes size, albeit GDF11 quantification methods have been recently questioned. In this study, we developed the IP based-PRM- LC-MS/MS method for measuring the GDF8 and GDF11 in one assay. The method demonstrated discriminatory power and allow the quantification of both proteins. Comparison of young and old group of mice show declination of both, GDF8 and GDF11 during aging. |
HostingRepository | PanoramaPublic |
AnnounceDate | 2021-01-24 |
AnnouncementXML | Submission_2021-01-24_16:42:07.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Olga Shevchuk |
SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
ModificationList | Carbamidomethyl; Label:13C(6)15N(4) |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2019-10-15 15:43:56 | ID requested | |
⏵ 1 | 2021-01-24 16:42:08 | announced |
Publication List
Camparini L, Kollipara L, Sinagra G, Loffredo FS, Sickmann A, Shevchuk O, Targeted Approach to Distinguish and Determine Absolute Levels of GDF8 and GDF11 in Mouse Serum. Proteomics, 20(11):e1900104(2020) [pubmed] |
Keyword List
submitter keyword: Parallel Reaction Monitoring (PRM), targeted, quantitative proteomics, Myostatin/GDF8, GDF11 |
Contact List
Olga Shevchuk | |
---|---|
contact affiliation | ISAS e.V. Otto-Hahn-Straße 6b 44227 Dortmund, Germany |
contact email | olga.shevchuk@isas.de |
lab head | |
Olga Shevchuk | |
contact affiliation | ISAS e.V. Otto-Hahn-Straße 6b 44227 Dortmund, Germany |
contact email | olga.shevchuk@isas.de |
dataset submitter |
Full Dataset Link List
Panorama Public dataset URI |