PXD015823-1

PXD015823 is an original dataset announced via ProteomeXchange.

Title	The RNA-binding protein ATXN2 controls cellular processes during megakaryopoiesis
Description	Megakaryocytes accumulate mRNA during their maturation, which is required for the correct spatio-temporal production of cytoskeletal proteins, membranes and platelet-specific granules, and for the subsequent shedding of thousands of platelets per cell. Gene expression profiling identified the RNA binding protein ATAXIN2 (ATXN2) as a putative novel regulator of megakaryopoiesis. ATXN2 expression is high in CD34+/CD41+ megakaryoblasts and sharply decreases upon maturation to megakaryocytes. ATXN2 associates with DDX6 suggesting that it may mediate repression of mRNA translation during early megakaryopoiesis. Comparative transcriptome and proteome analysis on megakaryoid cells (MEG-01) with differential ATXN2 expression identified ATXN2 dependent gene expression of mRNA and protein involved in processes linked to coagulation. Mice deficient for Atxn2 did not display differences in bleeding times, but expression of key surface receptors on platelets, such as ITGB3 (carries the CD61 antigen) and CD31 (PECAM1), were deregulated and platelet aggregation was reduced upon specific triggers.
HostingRepository	PRIDE
AnnounceDate	2020-05-26
AnnouncementXML	Submission_2020-05-26_15:05:58.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Arie Hoogendijk
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-15 01:21:40	ID requested
⏵ 1	2020-05-26 15:06:02	announced
2	2024-10-22 05:05:32	announced	2024-10-22: Updated project metadata.

submitter keyword: LC-MS, megakaryopoiesis

M. van den Biggelaar, PhD
contact affiliation	Department of Molecular and Cellular Hemostasis Plasma Proteins, Sanquin Research, Amsterdam, The Netherlands
contact email	m.vandenbiggelaar@sanquin.nl
lab head
Arie Hoogendijk
contact affiliation	Department of Plasma Proteins, Sanquin Research, Amsterdam
contact email	a.hoogendijk@sanquin.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015823
     1. Label: PRIDE project
     2. Name: The RNA-binding protein ATXN2 controls cellular processes during megakaryopoiesis