PXD015786 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Herpesvirus infection reduces Pol II occupancy of host promoters but spares viral promoters |
Description | In mammalian cells, widespread acceleration of cytoplasmic mRNA degradation is linked to impaired RNA polymerase II (Pol II) transcription. This mRNA decay-induced transcriptional repression occurs during infection with gammaherpesviruses including Kaposi’s sarcoma associated herpesvirus (KSHV) and murine gammaherpesvirus 68 (MHV68), which encode an mRNA endonuclease that initiates widespread RNA decay. Here, we show that MHV68-induced mRNA decay leads to a genome-wide reduction of Pol II occupancy at mammalian promoters. Viral genes, despite the fact that they require Pol II for transcription, escape this transcriptional repression. Protection is not governed by viral promoter sequences; instead, location on the viral genome is both necessary and sufficient to escape the transcriptional repression effects of mRNA decay. We hypothesize that the ability to escape from transcriptional repression is linked to the localization of viral DNA in replication compartments, providing a means for these viruses to counteract decay-induced viral transcript loss. |
HostingRepository | PRIDE |
AnnounceDate | 2019-12-10 |
AnnouncementXML | Submission_2019-12-10_06:27:58.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Joel Federspiel |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-10-11 01:17:27 | ID requested | |
⏵ 1 | 2019-12-10 06:27:59 | announced | |
2 | 2020-02-17 21:08:08 | announced | 2020-02-18: Updated publication reference for PubMed record(s): 32032393. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: MHV68, TMT, Herpesvirus, Infection, RNA Pol II |
Contact List
Ileana M Cristea |
contact affiliation | Department of Molecular Biology, Princeton University, USA |
contact email | icristea@princeton.edu |
lab head | |
Joel Federspiel |
contact affiliation | Princeton University |
contact email | joel.federspiel@princeton.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD015786 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD015786
- Label: PRIDE project
- Name: Herpesvirus infection reduces Pol II occupancy of host promoters but spares viral promoters