PXD015760-1

PXD015760 is an original dataset announced via ProteomeXchange.

Title	PRM of Nth1 phospho-peptides in response to AGC-kinase inhibition
Description	Protein phosphorylation cascades play a central role in the regulation of cell growth and protein kinases PKA, Sch9 and Ypk1 take centre stage in regulating this process in S. cerevisiae. To understand how these kinases co-ordinately regulate cellular functions we compared the phospho-proteome of exponentially growing cells without and with acute chemical inhibition of PKA, Sch9 and Ypk1. Reduced phosphorylation of Nth1 S83 upon PKA- and Ypk1-inhibition was validated by Western blotting and PRM.
HostingRepository	PanoramaPublic
AnnounceDate	2020-03-22
AnnouncementXML	Submission_2020-03-22_23:20:45.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Michael Plank
SpeciesList	scientific name: Saccharomyces cerevisiae; NCBI TaxID: 4932;
ModificationList	Phospho; Oxidation
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-09 09:18:44	ID requested
⏵ 1	2020-03-22 23:20:47	announced

Plank M, Perepelkina M, M, ü, ller M, Vaga S, Zou X, Bourgoint C, Berti M, Saarbach J, Haesendonckx S, Winssinger N, Aebersold R, Loewith R, Chemical Genetics of AGC-kinases Reveals Shared Targets of Ypk1, Protein Kinase A and Sch9. Mol Cell Proteomics, 19(4):655-671(2020) [pubmed]

submitter keyword: AGC-kinases, Sch9, Ypk1, PKA, chemical genetics

Michael Plank
contact affiliation	University of Geneva
contact email	michael.plank@unige.ch
lab head
Michael Plank
contact affiliation	University of Geneva
contact email	michael.plank@unige.ch
dataset submitter

Panorama Public dataset URI