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PXD015760 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePRM of Nth1 phospho-peptides in response to AGC-kinase inhibition
DescriptionProtein phosphorylation cascades play a central role in the regulation of cell growth and protein kinases PKA, Sch9 and Ypk1 take centre stage in regulating this process in S. cerevisiae. To understand how these kinases co-ordinately regulate cellular functions we compared the phospho-proteome of exponentially growing cells without and with acute chemical inhibition of PKA, Sch9 and Ypk1. Reduced phosphorylation of Nth1 S83 upon PKA- and Ypk1-inhibition was validated by Western blotting and PRM.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMichael Plank
SpeciesList scientific name: Saccharomyces cerevisiae; NCBI TaxID: 4932;
ModificationListPhospho; Oxidation
InstrumentOrbitrap Fusion ETD
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-10-09 09:18:44ID requested
12020-03-22 23:20:47announced
Publication List
Plank M, Perepelkina M, M, ΓΌ, ller M, Vaga S, Zou X, Bourgoint C, Berti M, Saarbach J, Haesendonckx S, Winssinger N, Aebersold R, Loewith R, Chemical Genetics of AGC-kinases Reveals Shared Targets of Ypk1, Protein Kinase A and Sch9. Mol Cell Proteomics, 19(4):655-671(2020) [pubmed]
Keyword List
submitter keyword: AGC-kinases, Sch9, Ypk1, PKA, chemical genetics
Contact List
Michael Plank
contact affiliationUniversity of Geneva
contact emailmichael.plank@unige.ch
lab head
Michael Plank
contact affiliationUniversity of Geneva
contact emailmichael.plank@unige.ch
dataset submitter
Full Dataset Link List
Panorama Public dataset URI