PXD015748-2

PXD015748 is an original dataset announced via ProteomeXchange.

Title	Mapping the HLA ligandome landscape of acute myeloid leukemia: a targeted approach toward peptide-based immunotherapy
Description	Identification of physiologically relevant peptide vaccine targets calls for the direct analysis of the entirety of naturally presented human leukocyte antigen (HLA) ligands, termed the HLA ligandome. In this study, we implemented this direct approach using immunoprecipitation and mass spectrometry to define acute myeloid leukemia (AML)-associated peptide vaccine targets. Mapping the HLA class I ligandomes of 15 AML patients and 35 healthy controls, more than 25 000 different naturally presented HLA ligands were identified. Target prioritization based on AML exclusivity and high presentation frequency in the AML cohort identified a panel of 132 LiTAAs (ligandome-derived tumor-associated antigens), and 341 corresponding HLA ligands (LiTAPs (ligandome-derived tumor-associated peptides)) represented subset independently in >20% of AML patients. Functional characterization of LiTAPs by interferon-γ ELISPOT (Enzyme-Linked ImmunoSpot) and intracellular cytokine staining confirmed AML-specific CD8+ T-cell recognition. Of note, our platform identified HLA ligands representing several established AML-associated antigens (e.g. NPM1, MAGED1, PRTN3, MPO, WT1), but found 80% of them to be also represented in healthy control samples. Mapping of HLA class II ligandomes provided additional CD4+ T-cell epitopes and potentially synergistic embedded HLA ligands, allowing for complementation of a multipeptide vaccine for the immunotherapy of AML.
HostingRepository	PRIDE
AnnounceDate	2019-10-14
AnnouncementXML	Submission_2019-10-16_07:51:48.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Annika Nelde
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-08 23:20:28	ID requested
1	2019-10-14 02:23:02	announced
⏵ 2	2019-10-16 07:51:50	announced	2019-10-16: Updated publication reference for PubMed record(s): 25092142.
3	2019-11-27 06:27:46	announced	2019-11-27: Updated project metadata.
4	2024-10-22 04:05:44	announced	2024-10-22: Updated project metadata.

Berlin C, Kowalewski DJ, Schuster H, Mirza N, Walz S, Handel M, Schmid-Horch B, Salih HR, Kanz L, Rammensee HG, Stevanovi, ć S, Stickel JS, Mapping the HLA ligandome landscape of acute myeloid leukemia: a targeted approach toward peptide-based immunotherapy. Leukemia, 29(3):647-59(2015) [pubmed]

submitter keyword: LC-MSMS, acute myeloid leukemia

Juliane S. Walz (neé Stickel)
contact affiliation	Department of Hematology and Oncology, University of Tübingen, Tübingen, Germany
contact email	juliane.walz@med.uni-tuebingen.de
lab head
Annika Nelde
contact affiliation	Department of Hematology and Oncology, University of Tübingen, Germany and Institute for Cell Biology, Department of Immunology, University of Tübingen, Germany
contact email	annika.nelde@uni-tuebingen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/10/PXD015748

PRIDE project URI

• PRIDE
  1. PXD015748
     1. Label: PRIDE project
     2. Name: Mapping the HLA ligandome landscape of acute myeloid leukemia: a targeted approach toward peptide-based immunotherapy