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PXD015727 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTissue-infiltrating macrophages mediate an exosome-based metabolic reprogramming upon DNA damage
DescriptionDNA damage and metabolic disorders are intimately linked with premature disease onset but the underlying mechanisms remain poorly understood. Here, we show that persistent DNA damage accumulation in tissue-infiltrating macrophages carrying an ERCC1-XPF DNA repair defect (Er1F/-) riggers Golgi dispersal, dilation of endoplasmic reticulum, autophagy and exosome biogenesis leading to the secretion of extracellular vesicles (EVs) in vivo and ex vivo. Macrophage-derived EVs accumulate in Er1F/- animal sera and are secreted in macrophage media after DNA damage. The Er1F/- EV cargo is taken up by recipient cells leading to an increase in insulin-independent glucose transporter levels, enhanced cellular glucose uptake, higher cellular oxygen consumption rate and greater tolerance to glucose challenge in mice. We find that high glucose in EV-targeted cells triggers pro-inflammatory stimuli via mTOR activation. This, in turn, establishes chronic inflammation and tissue pathology in mice with important ramifications for DNA repair-deficient, progeroid syndromes and aging.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMichalis Aivaliotis
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListOxidation; Acetyl; Carbamidomethyl
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-10-07 02:38:17ID requested
12019-11-06 02:28:39announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Mouse, nLC-MS/MS, Orbitrap, Proteome Discoverer, Scaffold, Label-free
Contact List
Michalis Aivaliotis
contact affiliationDirector of Proteomics Facility at IMBB (ProFI)
contact emailaivaliot@imbb.forth.gr
lab head
Michalis Aivaliotis
contact affiliationIMBB-FORTH/AUTH
contact emailaivaliot@imbb.forth.gr
dataset submitter
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Dataset FTP location
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