PXD015697-1

PXD015697 is an original dataset announced via ProteomeXchange.

Title	MALDI TOF/TOF of Delonix regia pollen protein
Description	Purpose: Delonix regia or Gulmohor commonly grows here and there in Indian villages as well as it is used for megacity beautification and environmental management due to its evergreen nature and vibrant flower colour. However, an increasing incidence of seasonal pollinosis was observed among the inhabitants living in close vicinity to this tree suggesting a possible link between the airborne pollen load and the concomitant respiratory hazards. This prompted us to investigate the allergens in the pollen of this dominant avenue tree. Methodology: Allergenicity of D. regia pollen grains was first checked by Skin Prick Test (SPT) and further confirmed by in vitro tests, such as, ELISA, Dot Blot and histamine release assay. The total proteome profiling was done by 2D PAGE and it was confronted with the pooled sera of 10 patients. The IgE reactive proteins were identified by MALDI TOF/TOF in Autoflex speed (Bruker,Germany). The raw spectra were searched against NCBInr database using MASCOT search engine. Result: Delonix regia, pollen grains have been found in considerable amount in the air during its flowering season (May to July). Approximately 31% of atopic individuals were found allergic to D. regia, pollen with elevated level of specific IgE and histamine in the serum. Total 8 IgE reactive proteins have been identified by homology driven proteomics. These proteins are ATP synthase beta subunit (spot no 5), Actin (spot no 6), Hypothetical actin like protein (spot no 7), Recombinant S-adenosylmethionine synthase 2 (spot no 9), UDP-arabinopyranose mutase (spot no 13), Luminal-binding 5 (spot no 2), ELF3-like protein (spot no 8) and hypothetical protein OsJ_04810 (spot no 11). Among these eight identified allergenic proteins, five were previously reported as allergen from different sources whereas the rest three have been reported for the first time as novel allergens. Conclusion: Novelty of this study was to identify 8 allergens from D.regia for the first time using immune-biochemical and proteomic techniques. Further studies will open up new avenues in component resolved diagnosis of pollen allergy.
HostingRepository	PRIDE
AnnounceDate	2021-09-08
AnnouncementXML	Submission_2021-09-08_14:25:23.251.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nandini Ghosh
SpeciesList	scientific name: Delonix regia; NCBI TaxID: 72433;
ModificationList	iodoacetamide derivatized residue
Instrument	autoflex

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-04 01:33:53	ID requested
⏵ 1	2021-09-08 14:25:23	announced
2	2024-10-22 05:26:54	announced	2024-10-22: Updated project metadata.

Bhowmik M, Ghosh N, Gupta Bhattacharya S, pollen grain and identification of allergens by immunoproteomic approach. Heliyon, 7(2):e06014(2021) [pubmed]

submitter keyword: Delonix, pollen, protein, mass spectrometry

Prof Swati Gupta Bhattacharya
contact affiliation	Professor, Division of Plant Biology,Bose Institute, Kolkata, India.
contact email	swati@jcbose.ac.in
lab head
Nandini Ghosh
contact affiliation	Vidyasagar University, Midnapore, West Bengal, India
contact email	nandini.ghosh14@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015697
     1. Label: PRIDE project
     2. Name: MALDI TOF/TOF of Delonix regia pollen protein