PXD015680 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Dissecting the antibacterial activity of Oxadiazolone-core derivatives against Mycobacterium abscessus |
Description | Mycobacterium abscessus is nowadays under the spotlight of the scientific community. This pathogenic mycobacteria is indeed responsible for a wide spectrum of infections involving mostly pulmonary infections in patients with cystic fibrosis. M. abscessus is intrinsically resistant to a broad range of antibiotics, including most antitubercular drugs, and is considered the most pathogenic and chemotherapy-resistant rapidly growing mycobacterium. Consequently, with very limited treatment options, the development of new therapeutic approaches to fight this pathogen are urgently needed. In this context, 19 oxadiazolone (OX) derivatives have been investigated for their antibacterial activity against both the rough (R) and smooth (S) variants of M. abscessus. Several OXs were active against extracellular M. abscessus growth with moderated minimal inhibitory concentrations (MIC), or intracellularly by inhibiting M. abscessus growth inside infected macrophages with MIC values similar to those of imipenem. Such promising results prompted us to identify the potential target enzymes of the sole extra and intracellular inhibitor of M. abscessus growth, i.e., iBpPPOX via activity-based protein profiling combined with mass spectrometry. This approach led to the identification of 21 potential protein candidates being mostly involved in M. abscessus lipid metabolism and/or in cell wall biosynthesis. |
HostingRepository | PRIDE |
AnnounceDate | 2020-08-14 |
AnnouncementXML | Submission_2020-09-27_23:53:34.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Luc Camoin |
SpeciesList | scientific name: Mycobacterium abscessus; NCBI TaxID: 36809; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-10-02 05:08:01 | ID requested | |
1 | 2020-08-14 02:11:38 | announced | |
⏵ 2 | 2020-09-27 23:53:35 | announced | 2020-09-28: Updated publication reference for PubMed record(s): 32946441. |
Publication List
Madani A, Mallick I, Guy A, Crauste C, Durand T, Fourquet P, Audebert S, Camoin L, Canaan S, Cavalier JF, Dissecting the antibacterial activity of oxadiazolone-core derivatives against Mycobacterium abscessus. PLoS One, 15(9):e0238178(2020) [pubmed] |
Keyword List
submitter keyword: Drug susceptibility |
intracellular activity |
activity based-protein profiling |
proteomic analysis |
Contact List
Luc Camoin |
contact affiliation | Aix Marseille Univ, Inserm, CNRS, Institut Paoli-Calmettes, CRCM, Marseille Protéomique & Aix Marseille Univ, CNRS, LISM, Institut de Microbiologie de la Méditerranée |
contact email | luc.camoin@inserm.fr |
lab head | |
Luc Camoin |
contact affiliation | Life Sciences |
contact email | luc.camoin@inserm.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD015680
- Label: PRIDE project
- Name: Dissecting the antibacterial activity of Oxadiazolone-core derivatives against Mycobacterium abscessus