PXD015679-3

PXD015679 is an original dataset announced via ProteomeXchange.

Title	membrane substrates of gingipains
Description	Porphyromonas gingivalis secretes cysteine proteases named gingipains which can cleave an array of proteins and importantly contribute to the development of periodontitis. In this study we focused on gingipain-exerted proteolysis at the cell surface of human gingival epithelial cells (telomerase immortalized gingival keratinocytes [TIGK]). We examined whether gingipains have sheddase activity or if their main activity is degradation of membrane proteins into small fragments. Using mass spectrometry, we investigated the whole sheddome/degradome of TIGK cell surface proteins by P. gingivalis strains differing in gingipain expression. We observed extensive degradation of TIGK surface proteins, suggesting that gingipains could in fact be the major cause of damage to the gingival epithelium. Most of the identified gingipain substrates were molecules involved in adhesion, suggesting that gingipains may cause tissue damage through cleavage of cell contacts, resulting in cell detachment and rounding, and consequently leading to anoikis. These results reveal a molecular underpinning to P. gingivalis-induced tissue destruction and enhance our knowledge of the role of P. gingivalis’ proteases in the pathobiology of periodontitis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:02:44.170.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marko Fonovic
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-02 05:07:58	ID requested
1	2020-04-28 04:14:21	announced
2	2020-07-15 00:43:52	announced	2020-07-15: Updated publication reference for PubMed record(s): 32411104.
⏵ 3	2024-10-22 05:02:51	announced	2024-10-22: Updated project metadata.

10.3389/fmicb.2020.00722;

Ho, č, evar K, Vizovi, š, ek M, Wong A, Kozie, ł J, Fonovi, ć M, Potempa B, Lamont RJ, Potempa J, Turk B, - Proteomic Insights Into Mechanisms Behind Tissue Damage in the Diseased Gingiva. Front Microbiol, 11():722(2020) [pubmed]

submitter keyword: protease, shedding, gingipain, anoikis, proteolysis

Marko Fonovic
contact affiliation	Department of Biochemistry and Molecular and Structural Biology, Jozef Stefan Institute, Ljubljana, Slovenia
contact email	marko.fonovic@ijs.si
lab head
Marko Fonovic
contact affiliation	Jozef Stefan Institute
contact email	marko.fonovic@ijs.si
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD015679

PRIDE project URI

• PRIDE
  1. PXD015679
     1. Label: PRIDE project
     2. Name: membrane substrates of gingipains