PXD015669 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | DirectMS1: MS/MS-free identification of 1000 proteins of cellular proteomes in 5 minutes |
Description | Proteome characterization relies heavily on tandem mass spectrometry (MS/MS) and is thus associated with instrumentation complexity, lengthy analysis time, and limited duty-cycle. It was always tempting to implement approaches which do not require MS/MS, yet, they were constantly failing in achieving meaningful depth of quantitative proteome coverage within short experimental times, which is particular important for clinical or biomarker discovery applications. Here, we report on the first successful attempt to develop a truly MS/MS-free and label-free method for bottom-up proteomics. We demonstrate identification of 1000 protein groups for a standard HeLa cell line digest using 5-minute LC gradients. The amount of loaded sample was varied in a range from 1 ng to 500 ng, and the method demonstrated 10-fold higher sensitivity compared with the standard MS/MS-based approach. Due to significantly higher sequence coverage obtained by the developed method, it outperforms all popular MS/MS-based label-free quantitation approaches |
HostingRepository | PRIDE |
AnnounceDate | 2020-02-27 |
AnnouncementXML | Submission_2020-02-27_01:00:06.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Mark Ivanov |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-10-02 01:11:39 | ID requested | |
⏵ 1 | 2020-02-27 01:04:34 | announced | |
Publication List
Ivanov MV, Bubis JA, Gorshkov V, Tarasova IA, Levitsky LI, Lobas AA, Solovyeva EM, Pridatchenko ML, Kjeldsen F, Gorshkov MV, DirectMS1: MS/MS-Free Identification of 1000 Proteins of Cellular Proteomes in 5 Minutes. Anal Chem, 92(6):4326-4333(2020) [pubmed] |
Keyword List
submitter keyword: Mass Spectrometry, Protein Identification, DDA, MS1-only, QExactive HF, HeLa, Yeast |
Contact List
Mikhail Vladimirovich Gorshkov |
contact affiliation | V. L. Talrose Institute for Energy Problems of Chemical Physics, N. N. Semenov Federal Research Center of Chemical Physics, Russian Academy of Sciences, 119334 Moscow, Russia |
contact email | mike.gorshkov@gmail.com |
lab head | |
Mark Ivanov |
contact affiliation | INEP CP RAS |
contact email | markmipt@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD015669
- Label: PRIDE project
- Name: DirectMS1: MS/MS-free identification of 1000 proteins of cellular proteomes in 5 minutes