PXD015623 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative proteomics of iron limitation in Klebsiella pneumoniae |
Description | Nutrient adaptation is key in limiting environments for the promotion of microbial growth and survival. In microbial systems, iron is an essential component for many cellular processes and bioavailability varies greatly among different conditions. In the bacterium, Klebsiella pneumoniae, the impact of iron limitation is known to alter transcriptional expression of iron-acquisition pathways and influences the secretion of iron-binding siderophores; however, a comprehensive view of iron limitation at the protein-level remains to be defined. Here, we apply a mass spectrometry-based quantitative proteomics strategy to profile the global impact of iron limitation on the cellular proteome and extracellular environment (secretome) of K. pneumoniae. Our data defines the impact of iron on proteins involved in transcriptional regulation and emphasizes the modulation of a vast array of proteins associated with iron acquisition, transport, and binding. We also identify proteins in the extracellular environment associated with conventional and nonconventional modes of secretion, as well as vesicle release. In particular, we demonstrate a new role for Lon protease in promoting iron homeostasis outside of the cell. Our characterization of Lon protease in K. pneumoniae validates roles in bacterial growth, cell division, iron utilization, and virulence. Moreover, our results uncover novel degradation candidates of Lon protease. Overall, we provide evidence of novel connections between Lon and iron in a bacterial system and define a unique role for Lon protease in the extracellular environment during nutrient limitation. |
HostingRepository | PRIDE |
AnnounceDate | 2020-03-09 |
AnnouncementXML | Submission_2020-03-09_00:56:41.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jennifer Geddes-McAlister |
SpeciesList | scientific name: Klebsiella pneumoniae; NCBI TaxID: 573; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-09-27 08:21:34 | ID requested | |
⏵ 1 | 2020-03-09 00:56:42 | announced | |
2 | 2020-06-12 02:35:26 | announced | 2020-06-12: Updated publication reference for PubMed record(s): 32390954. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Quantitative proteomics, iron limitation, Klebsiella pneumoniae, secretome, Lon protease |
Contact List
Jennifer Geddes-McAlister |
contact affiliation | Assistant Professor, Molecular and Cellular Biology Departmetn, University of Guelph |
contact email | jgeddesm@uoguelph.ca |
lab head | |
Jennifer Geddes-McAlister |
contact affiliation | University of Guelph |
contact email | jgeddesm@uoguelph.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD015623
- Label: PRIDE project
- Name: Quantitative proteomics of iron limitation in Klebsiella pneumoniae