PXD015605 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Systems-wide understanding of the osmotic response and metabolic rearrangement in Bacillus megaterium |
Description | For many years now, Bacillus megaterium has served as a microbial industrial strain for high-level production of recombinant proteins in the g/L-scale. Nevertheless, the impact of process-related stress has only been poorly characterized so far. Taking advantage of the recent technical developments for quantifying the cell at various molecular levels, we interrogated the osmotic stress response of B. megaterium using transcriptome, proteome, metabolome and fluxome analyses. Under osmotic upshift conditions, several stress response enzymes, iron scavenging, and reactive oxygen species (ROS) fighting proteins were upregulated. The downregulation of genes of the upper part of glycolysis resulted in the activation of the pentose phosphate pathway (PPP), generating an oversupply of NADPH. The (NADH/NAD+) ratio indicating the redox state of the cell was also altered, which was partially compensated by the higher production of lactate accompanied by the reduction of acetate secretion. NADH was produced mainly within the tricarboxylic acid cycle (TCA) cycle elucidated from the higher mRNA and protein levels of enzymes involved within this pathway. This adaptation mainly focused on the massive de novo synthesis of the compatible solute proline recruiting an osmo-dependent pathway to fulfil this requirement. 13C flux analyses confirmed these findings. Giving the high flux towards acetyl-CoA and large pool of NADPH, B. megaterium cells redirected the produced acetyl-CoA to the polyhydroxybutyrate (PHB) biosynthetic pathway under non-limiting nutrient condition, amassing around 30% of the CDW as PHB. This direct relation between osmotic stress and intracellular PHB content has been evidenced for the first time, thus opening new avenues for synthesizing this valuable biopolymer using varying salt concentrations under non-limiting nutrient conditions. |
HostingRepository | PRIDE |
AnnounceDate | 2020-05-26 |
AnnouncementXML | Submission_2020-05-26_14:51:45.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Daniela Zuehlke |
SpeciesList | scientific name: Bacillus megaterium (strain DSM 319); NCBI TaxID: 592022; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Synapt MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-09-26 02:17:40 | ID requested | |
⏵ 1 | 2020-05-26 14:51:46 | announced | |
Publication List
Godard T, Z, ΓΌ, hlke D, Richter G, Wall M, Rohde M, Riedel K, Poblete-Castro I, Krull R, Biedendieck R, . Front Bioeng Biotechnol, 8():47(2020) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Bacillus megaterium, osmotic stress, transcriptomics, proteomics, flux analysis, proline, polyhydroxybutyrate |
Contact List
Katharina Riedel |
contact affiliation | University of Greifswald, Institute of Microbiology, Department of Microbial Physiology and Molecular Biology, Greifswald, Germany |
contact email | riedela@uni-greifswald.de |
lab head | |
Daniela Zuehlke |
contact affiliation | Ernst Moritz Arndt University Greifswald, Institute of Microbiology |
contact email | zuehlke@uni-greifswald.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD015605 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD015605
- Label: PRIDE project
- Name: Systems-wide understanding of the osmotic response and metabolic rearrangement in Bacillus megaterium