PXD015592-1

PXD015592 is an original dataset announced via ProteomeXchange.

Title	Enzymatic dissociation induces transcriptional and proteotype bias in brain cell populations
Description	Different cell isolation techniques exist for transcriptomic and proteotype profiling of brain cells. Here we show that standard enzymatic digestion of brain tissue at 37°C induces profound alterations in the transcriptome and proteotype of specific brain cells, as compared to cold mechanical dissociation. These findings emphasize the need to consider intrinsic bias and biological artefacts when implementing enzymatic digestion-based isolation methods for brain cell analyses.
HostingRepository	PRIDE
AnnounceDate	2022-02-15
AnnouncementXML	Submission_2022-02-15_09:26:34.720.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marc van Oostrum
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-25 23:45:03	ID requested
⏵ 1	2022-02-15 09:26:35	announced

Mattei D, Ivanov A, van Oostrum M, Pantelyushin S, Richetto J, Mueller F, Beffinger M, Schellhammer L, Vom Berg J, Wollscheid B, Beule D, Paolicelli RC, Meyer U, Enzymatic Dissociation Induces Transcriptional and Proteotype Bias in Brain Cell Populations. Int J Mol Sci, 21(21):(2020) [pubmed]

curator keyword: Biological

submitter keyword: Brain cell types enzymatic digestion bias isolation

Bernd Wollscheid
contact affiliation	Institute of Molecular Systems Biology and Department for Health Sciences and Technology, ETH Zürich, Zurich, Switzerland
contact email	wbernd@ethz.ch
lab head
Marc van Oostrum
contact affiliation	ETH Zürich
contact email	marcv@ethz.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015592
     1. Label: PRIDE project
     2. Name: Enzymatic dissociation induces transcriptional and proteotype bias in brain cell populations