PXD015584-1

PXD015584 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Quantitative Analysis of Global Protein Stability Rates in Tissues
Description	We have developed QUAD (Quantification of Azidohomoalanine Degradation), a technique to quantitate global protein degradation using mass spectrometry. Azidohomoalanine (AHA) is pulsed into mouse tissues through their diet. The mice are then returned to a normal diet and the decrease of AHA abundance can be quantitated in the proteome. QUAD analysis reveals that protein stability varied within tissues, but discernible trends in the data suggest that cellular environment is a major factor dictating stability. Within a tissue, different organelles, post-translation modifications, and protein functions were enriched with different stability patterns. Surprisingly, subunits of the TRIC molecular chaperonin possessed markedly distinct stability trajectories in the brain. Further investigation revealed that these subunits also possessed different subcellular localization indicating a potential non-canonical chaperonin. Finally, QUAD analysis demonstrated that protein stability is enhanced with age in the brain but not in the liver. Overall, QUAD allows the first global quantitation of protein stability rates in tissues, which may lead to new insights and hypotheses in basic and translational research.
HostingRepository	MassIVE
AnnounceDate	2020-09-08
AnnouncementXML	Submission_2020-09-08_13:57:05.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniel McClatchy
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	Met->Aha
Instrument	LTQ Velos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-25 09:32:44	ID requested
⏵ 1	2020-09-08 13:57:05	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Azidohomoalanine, PALM, AHA, degradation, QUAD

submitter keyword: Azidohomoalanine, PALM, AHA, degradation, QUAD

Contact List

John R. Yates III
contact affiliation	The Scripps Research Institute
contact email	jyates@scripps.edu
lab head
Daniel McClatchy
contact affiliation	TSRI
contact email	dmcclat@scripps.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000084376/