PXD015580-1

PXD015580 is an original dataset announced via ProteomeXchange.

Title	KRAS G12V digestion by 20S proteasome
Description	KRAS is a well known tumor associated antigen. It’s a GTPase that functions as molecular switch in regulatory pathways responsible for proliferation and survival. It’s frequently mutated in a variety of cancers. One of the possible driver mutations is G12V substitution, which impairs KRAS GTPase activity and renders the mutants persistently in the GTP-bound active form, thereby promoting tumorigenesis and tumor malignancy. Its high frequency in cancers makes KRAS an attractive target for immunotherapy. We performed in vitro digestions of synthetic polypeptide corresponding to KRAS2-35 carrying the mutation with purified 20S proteasome. Samples were measured by LC-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2019-10-24
AnnouncementXML	Submission_2019-10-24_02:05:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Artem Mansurkhodzhaev
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-25 06:09:35	ID requested
⏵ 1	2019-10-24 02:17:05	announced
2	2019-11-27 06:27:38	announced	2019-11-27: Updated project metadata.
3	2019-12-09 02:27:52	announced	2019-11-27: Updated project metadata. 2019-12-09: Updated publication reference for PubMed record(s): 31803176.
4	2024-10-22 04:58:07	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: proteasome, KRAS,cancer, digestion, LC-MS/MS

Juliane Liepe
contact affiliation	Head of research group Quantitative and Systems Biology; Max Planck Institute for Biophysical Chemistry; Goettingen; Germany
contact email	juliane.liepe@mpibpc.mpg.de
lab head
Artem Mansurkhodzhaev
contact affiliation	Max Planck Institute for Biophysical Chemistry
contact email	artem.mansurkhodzhaev@mpibpc.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/10/PXD015580

PRIDE project URI

• PRIDE
  1. PXD015580
     1. Label: PRIDE project
     2. Name: KRAS G12V digestion by 20S proteasome