PXD015565-1

PXD015565 is an original dataset announced via ProteomeXchange.

Title	SUMOylation regulates the metaphase-anaphase transition by stabilizing sister kinetochore biorientation
Description	The accurate segregation of chromosomes during mitosis relies on the attachment of sister chromatids to microtubules from opposite poles, called biorientation. Sister chromatid cohesion resists microtubule forces, generating tension which provides the signal that biorientation has occurred. How tension silences the surveillance pathways that prevent cell cycle progression and correct erroneous kinetochore-microtubule remains unclear. Here we identify SUMOylation as a mechanism that promotes anaphase onset upon biorientation. SUMO ligases modify the tension-sensing pericentromere-localized chromatin protein, shugoshin, to stabilize bioriented sister kinetochore-microtubule attachments and allow entry into anaphase. SUMOylation of shugoshin prevents binding to protein phosphatase 2A (PP2A), while enhancing chromosome passenger complex (CPC) interaction and removal from centromeres. Dissociation of the shugoshin-PP2A interaction is important for stabilizing sister kinetochore biorientation. Therefore, SUMOylation modulates protein interactions within pericentromeres to allow a signalling switch in response to biorientation.
HostingRepository	PRIDE
AnnounceDate	2021-05-06
AnnouncementXML	Submission_2021-05-06_07:50:24.572.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mike Tatham
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	sumoylated lysine; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-24 05:37:22	ID requested
⏵ 1	2021-05-06 07:50:24	announced
2	2021-05-06 22:10:47	announced	2021-05-07: Updated publication reference for PubMed record(s): 33929514.
3	2024-10-22 05:21:49	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Yeast SUMO SMT3 SGO1

Ronald T. Hay
contact affiliation	University of Dundee
contact email	r.t.hay@dundee.ac.uk
lab head
Mike Tatham
contact affiliation	University of Dundee
contact email	m.tatham@dundee.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015565
     1. Label: PRIDE project
     2. Name: SUMOylation regulates the metaphase-anaphase transition by stabilizing sister kinetochore biorientation