PXD015530-1
PXD015530 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Go_BioID_humancellmap_HEK293_lowSDS_2019 |
Description | This dataset consists of 68 raw MS files and associated peak lists and result files, acquired on Velos Orbitrap Elite mass spectrometer operated in Data Dependent Acquisition mode. All samples are BioID experiments, performed in HEK293 Flp-In T-REx cells from organellar and other subcellular markers using a protocol with low concentrations of SDS for lysis and washes. Sample preparation and mass spectrometric acquisition were performed by Christopher Go. The files are associated with a manuscript by Go et al. that describes generation of a proximity-dependent biotinylation (BioID) map of a human cell. Anne-Claude Gingras should be contacted for questions on this dataset (gingras@lunenfeld.ca). |
HostingRepository | MassIVE |
AnnounceDate | 2021-06-17 |
AnnouncementXML | Submission_2021-06-17_09:58:30.169.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Gingras Lab |
SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
ModificationList | deamidated L-asparagine; deamidated L-glutamine; L-methionine sulfoxide |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2019-09-22 00:25:59 | ID requested | |
⏵ 1 | 2021-06-17 09:58:31 | announced |
Publication List
Go CD, Knight JDR, Rajasekharan A, Rathod B, Hesketh GG, Abe KT, Youn JY, Samavarchi-Tehrani P, Zhang H, Zhu LY, Popiel E, Lambert JP, Coyaud É, Cheung SWT, Rajendran D, Wong CJ, Antonicka H, Pelletier L, Palazzo AF, Shoubridge EA, Raught B, Gingras AC, A proximity-dependent biotinylation map of a human cell. Nature, 595(7865):120-124(2021) [pubmed] |
Keyword List
submitter keyword: BioID, Proximity-dependent biotinylation, human cell map, organelle proteomics |
Contact List
Anne-Claude Gingras | |
---|---|
contact affiliation | LTRI |
contact email | gingras@lunenfeld.ca |
lab head | |
Gingras Lab | |
contact affiliation | Samuel Lunenfeld Research Institute |
contact email | gingras@mshri.on.ca |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000084359/ |