PXD015520-1

PXD015520 is an original dataset announced via ProteomeXchange.

Title	The long non-coding RNA HOXB-AS3 regulates ribosomal RNA transcription in NPM1-mutated acute myeloid leukemia
Description	In this work we dissect the functional role of the HOXB-AS3 long non coding RNA in patients with NPM1-mutated (NPM1mut) acute myeloid leukemia (AML). We show that HOXB-AS3 regulates the proliferative capacity of NPM1mut AML blasts in vitro and in vivo. HOXB-AS3 was found to interact with the ErbB3-binding protein 1 (EBP1) and guide EBP1 to the ribosomal DNA locus. Via this mechanism HOXB-AS3 regulates ribosomal RNA transcription and de novo protein synthesis. We propose that in the context of NPM1 mutations, HOXB-AS3 overexpression acts as a compensatory mechanism, which allows adequate protein production in leukemic blasts.
HostingRepository	PRIDE
AnnounceDate	2019-10-16
AnnouncementXML	Submission_2019-10-16_06:20:13.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christopher Walker
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-20 01:25:46	ID requested
⏵ 1	2019-10-16 06:20:14	announced

Dataset with its publication pending

submitter keyword: HOXB-AS3, lncRNA, EBP1, acute myeloid leukemia

Ramiro Garzon
contact affiliation	Associate Professor, Division of Hematology, Department of Internal Medicine, The Ohio State University Wexner Medical Center
contact email	Ramiro.Garzon@osumc.edu
lab head
Christopher Walker
contact affiliation	The Ohio State University
contact email	christopher.walker@osumc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/10/PXD015520

PRIDE project URI

• PRIDE
  1. PXD015520
     1. Label: PRIDE project
     2. Name: The long non-coding RNA HOXB-AS3 regulates ribosomal RNA transcription in NPM1-mutated acute myeloid leukemia