PXD015498-1

PXD015498 is an original dataset announced via ProteomeXchange.

Title	Characterization of the human myocardial phosphoproteome in aortic stenosis patients with complete or incomplete reverse remodeling
Description	Aortic valve stenosis (AS) is a serious condition characterized by the progressive narrowing of the aortic valve, associated with a continuous rise in left ventricle pressure overload. Currently, aortic valve replacement (AVR) remains as the definitive treatment for AS, since an immediate dissipation of left ventricle pressure overload is achieved with this intervention. Upon AVR, a myocardial response, known as reverse remodeling (RR), is set into motion, characterized by regression of hypertrophy and cardiac function normalization. In spite of the AVR benefits, cardiologists still face the problem of RR variability, with some patients displaying a complete and others an incomplete recovery, for instance, due to prevailing hypertrophy. Patients with incomplete RR are at higher risk of developing diastolic dysfunction and, ultimately, heart failure. Myocardial biopsies, available during AVR, provide a wealth of information through its molecular characterization. Particularly, the characterization of the myocardium phosphoproteome may help identify dysregulated pathways underlying an incomplete RR. Moreover, kinase prediction can also be useful to identify new molecular targets aiming at improving the phenotype of this subset of patients. Hence, we aimed to characterize, for the first time, the myocardial phosphoproteome from AS patients with complete and incomplete RR. To fulfil this task, we followed a shotgun LC-MS/MS approach using an Orbitrap instrument, after TiO2-based phosphopeptide enrichment. Phosphopeptides were identified and quantified through a MaxQuant label-free quantification approach (FDR 1%).
HostingRepository	PRIDE
AnnounceDate	2025-10-20
AnnouncementXML	Submission_2025-10-19_16:18:04.914.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fábio Trindade
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-18 05:37:14	ID requested
⏵ 1	2025-10-19 16:18:05	announced

10.1007/s00395-025-01125-w;

Trindade F, Almeida-Coelho J, Sousa-Mendes C, Saraiva F, Arbon, é, s ML, Leite-Moreira A, Vitorino R, Falc, ã, o-Pires I, Myocardial phosphoproteomics unveils a key role of DYRK1A in aortic valve replacement-induced reverse remodelling. Basic Res Cardiol, 120(5):947-974(2025) [pubmed]

submitter keyword: human

phosphoproteome

myocardium

aortic stenosis

reverse remodeling

Rui Vitorino
contact affiliation	iBiMED – Institute of Biomedicine, Department of Medical Sciences, University of Aveiro, Aveiro, Portugal; UnIC – Unidade de Investigação Cardiovascular, Departamento de Cirurgia e Fisiologia, Faculdade de Medicina da Universidade do Porto, Porto, Portugal
contact email	rvitorino@ua.pt
lab head
Fábio Trindade
contact affiliation	iBiMED, University of Aveiro; UnIC, Faculty of Medicine, University of Porto
contact email	fabiotrindade@ua.pt
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015498
     1. Label: PRIDE project
     2. Name: Characterization of the human myocardial phosphoproteome in aortic stenosis patients with complete or incomplete reverse remodeling