PXD015488-1

PXD015488 is an original dataset announced via ProteomeXchange.

Title	Gcn5 and Esa1 function as histone crotonyltransferases to regulate crotonylation-dependent transcription
Description	Histone post-translational modifications (PTMs) are critical for processes such as transcription. The more notable among these are the non-acetyl histone lysine acylation modifications such as crotonylation, butyrylation and succinylation. However, the biological relevance of these PTMs is not fully understood because their regulation is largely unknown. Here, we set out to investigate whether the main histone acetyltransferases in budding yeast, Gcn5 and Esa1, possess crotonyltransferase activity. In vitro studies revealed that the Gcn5-Ada2-Ada3 (ADA) and Esa1-Yng2-Epl1 (Piccolo NuA4) histone acetyltransferase complexes have the capacity to crotonylate histones. Mass spectrometry analysis revealed that ADA and Piccolo NuA4 crotonylate lysines in the N-terminal tails of histone H3 and H4, respectively. Functionally, we show that crotonylation selectively affects gene transcription in vivo in a manner dependent on Gcn5 and Esa1. Thus, we identify the Gcn5- and Esa1-containing ADA and Piccolo NuA4 complexes as bona fide crotonyltransferases that promote crotonylation-dependent transcription.
HostingRepository	PRIDE
AnnounceDate	2019-11-06
AnnouncementXML	Submission_2019-11-06_07:57:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	G Janssen
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	N6-crotonyl-L-lysine; acetylated residue
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-17 07:58:46	ID requested
⏵ 1	2019-11-06 07:57:53	announced
2	2024-10-22 04:57:49	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: epigenetics, histone modification, crotonylation, Gcn5-Ada2-Ada3 (ADA) complex, Esa1- Yng2-Epl1 (Piccolo NuA4) complex, gene transcription

P.A. van Veelen
contact affiliation	Dr. P.A. van Veelen LUMC Centre for Proteomics and Metabolomics Head Proteomics group
contact email	P.A.van_Veelen@lumc.nl
lab head
G Janssen
contact affiliation	Leiden University Medical Center
contact email	g.m.c.janssen@lumc.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015488
     1. Label: PRIDE project
     2. Name: Gcn5 and Esa1 function as histone crotonyltransferases to regulate crotonylation-dependent transcription