PXD015476 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Yeast homologs of MCUR1 regulate mitochondrial proline metabolism |
Description | MCUR1 is an evolutionarily conserved mitochondrial protein that has been shown to act as a scaffold factor for the assembly of the mammalian mitochondrial calcium uniporter (MCU). Surprisingly, many fungi that do not have MCU contain MCUR1 homologs, suggesting these proteins possess alternate functions. Herein we characterized two Saccharomyces cerevisiae homologs of MCUR1- Put6 and Put7 as regulators of mitochondrial proline metabolism. Put6 and Put7 are tethered to the inner mitochondrial membrane in a large hetero-oligomeric complex. The abundance and assembly of this complex are regulated by proline and the loss of this complex results in unregulated proline uptake in the mitochondria, leading to redox imbalance in the cell. Yeast cells lacking either Put6 or Put7 exhibit a pronounced defect in proline utilization, which can be corrected by the heterologous expression of human MCUR1. Our work uncovers an unexpected role of evolutionarily conserved MCUR1 homologs in mitochondrial proline metabolism. |
HostingRepository | PRIDE |
AnnounceDate | 2020-09-30 |
AnnouncementXML | Submission_2020-09-29_23:32:02.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Marta Murgia |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-09-17 01:54:03 | ID requested | |
⏵ 1 | 2020-09-29 23:32:02 | announced | |
Publication List
Zulkifli M, Neff JK, Timbalia SA, Garza NM, Chen Y, Watrous JD, Murgia M, Trivedi PP, Anderson SK, Tomar D, Nilsson R, Madesh M, Jain M, Gohil VM, Yeast homologs of human MCUR1 regulate mitochondrial proline metabolism. Nat Commun, 11(1):4866(2020) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: mitochondrion, proline metabolism, MCUR1 |
Contact List
Vishal M. Gohil |
contact affiliation | Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843, USA |
contact email | vgohil@tamu.edu |
lab head | |
Marta Murgia |
contact affiliation | MPI Biochemistry |
contact email | mmurgia@biochem.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD015476
- Label: PRIDE project
- Name: Yeast homologs of MCUR1 regulate mitochondrial proline metabolism