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PXD015471-2

PXD015471 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe mRNA-binding proteome of proliferating and differentiated muscle cells
DescriptionMuscle formation is a coordinated process driven by extensive gene expression changes where single cells fuse together to form multinucleated muscle fibers. Newly synthesized mRNAs are then regulated by RNA binding proteins(RBPs)affecting post-transcriptional transcripts metabolism. We determined how large gene expression changes affect the catalog of RBPs by studying proliferating and differentiated muscle cells in healthy and dystrophic conditions. Transcriptomic analysis showed that the expression of more than 7000 genes was affected during myogenesis. We identified 769 RBPs, of which 294 were muscle specific and 49 were uniquely shared with cardiomyocytes. A subset of 32 RBPs (half of which was muscle specific) showed preferential loading on target mRNAs in either myoblasts or myotubes. A large proportion of catalytic proteins was bound to mRNAs even though they lack classical RNA binding domains. Finally we show how the identification of cell specific RBPs enabled the identification biomarkers that can separate healthy individuals from dystrophic patients. Our data show how interactome data can shed light on new basic RNA biology as well as provide cell specific data that can be used for diagnostic purposes.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:06:55.215.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterG Janssen
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-09-16 07:30:54ID requested
12020-06-08 17:00:49announced
22024-10-22 05:06:55announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biomedical
submitter keyword: RNA binding proteins
Interactome
Duchenne muscular dystrophy
Contact List
P.A.van Veelen
contact affiliationCentre for Proteomics and Metabolomics Head Proteomics group LUMC
contact emailP.A.van_Veelen@lumc.nl
lab head
G Janssen
contact affiliationLeiden University Medical Center
contact emailg.m.c.janssen@lumc.nl
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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