PXD015461-1

PXD015461 is an original dataset announced via ProteomeXchange.

Title	Peroxisome elevation induces stem cell differentiation and intestinal epithelial repair
Description	To identify the regulators that directly interact with peroxisomes to promote the differentiation of intestinal stem and progenitor cells after injury, peroxisomes were isolated from both wild-type and Pex10-null flies and mass spectrometry (MS) analysis was performed. For peroxisomes isolation and mass spectrometry, 100 female 5-day-old wild-type or Pex10-null files without bleomycin treatment (BLM-0D WT, BLM-0D Pex10-null) or treatment with bleomycin for one day followed by recovery for one day (BLM-REC-1D WT, BLM-REC-1D Pex10-null) were homogenized. Each group repeat two times. The MS analysis was performed on Q ExactiveTM Mass Spectrometer (Thermo). The peptides were subjected to NSI source followed by tandem mass spectrometry (MS/MS) in Q ExactiveTM Mass Spectrometer coupled online to the HPLC. Intact peptides were detected in the Orbitrap at a resolution of 70,000 and peptides were selected for MS/MS using NCE setting as 27. Protein identification was performed with MASCOT software by searching Uniprot Drosophila melanogaster.
HostingRepository	iProX
AnnounceDate	2019-09-16
AnnouncementXML	Submission_2019-09-16_03:32:06.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gang Du
SpeciesList	scientific name: Drosophila melanogaster; NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-16 03:30:21	ID requested
⏵ 1	2019-09-16 03:32:07	announced
2	2020-11-30 00:09:35	announced	2020-11-30: Update publication information

Dataset with its publication pending

submitter keyword: stem cell differentiation

Haiyang Chen
contact affiliation	School of life science
contact email	chenhy87@mail.sysu.edu.cn
lab head
Gang Du
contact affiliation	School of life science
contact email	dugang19880421@126.com
dataset submitter

iProX dataset URI