PXD015447-1

PXD015447 is an original dataset announced via ProteomeXchange.

Title	FeS protein levels in Arabidopsis plants expressing a mutated NEET protein
Description	Iron-sulfur (Fe-S) clusters play an essential role in plants as protein co-factors mediating diverse electron transfer reactions. Because they can react with oxygen to form reactive oxygen species (ROS) and cause cellular damage, the biogenesis of Fe-S clusters is highly regulated. A newly discovered group of 2Fe-2S proteins, termed NEET proteins, was recently proposed to coordinate iron-sulfur, iron, and ROS homeostasis in mammalian cells. Here we report that disrupting the function of AtNEET, the sole member of the NEET protein family in Arabidopsis thaliana, triggers a leaf-associated Fe- and Fe-S-deficiency responses, iron overload in chloroplasts (1.2-1.5 fold), chlorosis, structural damage to chloroplasts, and a high seedling mortality rate. Our findings suggest that disrupting AtNEET function disrupts the transfer of 2Fe-2S clusters from the chloroplastic 2Fe-2S biogenesis pathway to Fe-S proteins, and that uncoupling this process triggers a leaf-associated Fe-deficiency response that results in iron overload in chloroplasts and enhanced ROS accumulation
HostingRepository	PanoramaPublic
AnnounceDate	2021-01-24
AnnouncementXML	Submission_2021-01-24_16:20:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sara I Zandalinas
SpeciesList	scientific name: Arabidopsis thaliana; NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	TSQ Quantiva

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-13 12:22:53	ID requested
⏵ 1	2021-01-24 16:20:41	announced

Zandalinas SI, Song L, Sengupta S, McInturf SA, Grant DG, Marjault HB, Castro-Guerrero NA, Burks D, Azad RK, Mendoza-Cozatl DG, Nechushtai R, Mittler R, Expression of a dominant-negative AtNEET-H89C protein disrupts iron-sulfur metabolism and iron homeostasis in Arabidopsis. Plant J, 101(5):1152-1169(2020) [pubmed]

submitter keyword: Arabidopsis, NEET, Fe-S

Ron Mittler
contact affiliation	University of Missouri
contact email	mittlerr@missouri.edu
lab head
Sara I Zandalinas
contact affiliation	University of Missouri
contact email	sara.i.zandalinas@gmail.com
dataset submitter

Panorama Public dataset URI