PXD015410 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome profiling of myogenic differentiation of alveolar mucosa multipotent mesenchymal stromal cells revealed an unexpected deviation between 2D monolayer and 3D spheroid model cultures. |
Description | Recently, it has been proposed to employ three-dimensional cultivation of cell in spheroids that contributes more proper physiological conditions in matrix extracellular space architecture and cell morphology accompanied by appropriate intracellular biomechanical organization of cytoskeleton and energy generation apparatus adaptation. In this research we presented the data of proteome survey for cells growing in monolayer and in spheroids that were induced in myogenic way of differentiation and reached terminal stage in seven days. Semi-quantitative profiling of the dynamic changes in proteins abundance depicted strict advantage of spheroid manner cultivation in contrast to monolayer. Cells cultured in spheroid were significantly enriched in proteins of mitochondria biogenesis, respiratory chain proteins, extracellular proteins and cytoskeleton. Most of signal transducers involved in active proliferation and early stages of differentiation were inhibited in spheroid cultures unlike monolayer. The obtained data were broadly consistent with known molecular mechanisms of myogenesis in transcripts level and reflected strong advantage of spheroid cultivation conditions. |
HostingRepository | PRIDE |
AnnounceDate | 2021-11-15 |
AnnouncementXML | Submission_2021-11-15_13:45:50.010.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD015410 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Arthur Kopylov |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | Glu->pyro-Glu; Deamidated; Oxidation; Acetyl; Pyridylethyl; Gln->pyro-Glu |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-09-11 03:36:41 | ID requested | |
⏵ 1 | 2021-11-15 13:45:50 | announced | |
Publication List
Saburina IN, Kosheleva NV, Kopylov AT, Lipina TV, Krasina ME, Zurina IM, Gorkun AA, Girina SS, Pulin AA, Kaysheva AL, Morozov SG, Proteomic and electron microscopy study of myogenic differentiation of alveolar mucosa multipotent mesenchymal stromal cells in three-dimensional culture. Proteomics, 22(3):e2000304(2022) [pubmed] |
Keyword List
submitter keyword: myogenesis |
monolayer culture |
spheroid culture |
cytoskeleton |
mitochondria |
autophagy; |
Contact List
Pulin Andrey |
contact affiliation | Laboratory for Cell Biology and Developmental Pathology, Institute of General Pathology and Pathophysiology, 8 Baltiyskaya st., Moscow, 125315 |
contact email | andreypulin@gmail.com |
lab head | |
Arthur Kopylov |
contact affiliation | Institute of Biomedical Chemistry |
contact email | a.t.kopylov@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD015410
- Label: PRIDE project
- Name: Proteome profiling of myogenic differentiation of alveolar mucosa multipotent mesenchymal stromal cells revealed an unexpected deviation between 2D monolayer and 3D spheroid model cultures.