PXD015374-2

PXD015374 is an original dataset announced via ProteomeXchange.

Title	Functional analysis of phytoplasma effectors in Vitis vinifera pathogenesis
Description	The aim of the experiment was to identify important protein targets of phytoplasma infection, by isolation of plant proteins, interacting with phytoplasma effectors. Nicotiana benthamiana plants were transformed with YFP (Yellow Fluorescent Protein) tagged phytoplasma effector PoStoSP28, the leaves of transformed plants were homogenized and the effector was extracted together with its interactors through the YFP tag with a pull-down assay. As a control, the same procedure was performed with mock transformed plants and the samples analyzed with mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:06:24.559.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stefane Wienkoop
SpeciesList	scientific name: Nicotiana tabacum (Common tobacco); NCBI TaxID: 4097;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-09 05:10:50	ID requested
1	2023-10-24 07:28:57	announced
⏵ 2	2023-11-14 09:06:25	announced	2023-11-14: Updated project metadata.
3	2024-10-22 06:07:27	announced	2024-10-22: Updated project metadata.

Dermastia M, Toma, ž Š, Strah R, Lukan T, Coll A, Du, š, ak B, An, ž, i, č B, Č, epin T, Wienkoop S, Kladnik A, Zagor, š, č, ak M, Riedle-Bauer M, Sch, ö, nhuber C, Weckwerth W, Gruden K, Roitsch T, Pompe Novak M, Brader G, Phytoplasma solani' modulate plant carbohydrate metabolism, accelerate the ascorbate-glutathione cycle, and induce autophagosomes. Front Plant Sci, 14():1232367(2023) [pubmed]

curator keyword: Biological

submitter keyword: Nicotiana benthamiana,phytoplasma effectors. Yellow Fluorescent Protein (YFP), Vitis vinifera, pathogenesis

Stefanie Wienkoop
contact affiliation	Devision of Molecular Systems Biology, University of Vienna, Austria
contact email	stefanie.wienkoop@univie.ac.at
lab head
Stefane Wienkoop
contact affiliation	Molecular Systems Biology
contact email	stefanie.wienkoop@univie.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD015374

PRIDE project URI

• PRIDE
  1. PXD015374
     1. Label: PRIDE project
     2. Name: Functional analysis of phytoplasma effectors in Vitis vinifera pathogenesis