PXD015364-2

PXD015364 is an original dataset announced via ProteomeXchange.

Title	A label-free shotgun proteomics analysis of macadamia nut
Description	In this study, we present a first proteomic overview of macadamia nut using a label-free shotgun proteomic approach and prediction of their proteins with potential allergenic activity and cross-reactivity via an in silico analysis. The defense response proteins were the most abundant group of proteins and accounted for 24% of the total abundance. The vicilin-like macadamia antimicrobial peptides 2-3 (MiAMP2) was the most abundant protein. The cascading of amino acid and carbohydrate metabolic pathways in macadamia nut were constructed and proposed for the first time. The in silico analysis revealed seed storage proteins (conglutins and vicilins), rubber elongation factor proteins, phosphate binding proteins, and detoxifying methylglyoxalases among others as proteins with allergenic and cross-reactive potential. The label-free shotgun proteomics study revealed valuable insight into the genetic and biological makeup of macadamia nut and potential proteins with predictive allergenic activity and cross-reactivity. Integration of genomics with the proteomic data will be indispensable for future molecular characterization of macadamia including allergens.
HostingRepository	PRIDE
AnnounceDate	2020-01-13
AnnouncementXML	Submission_2020-01-15_06:12:10.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	sridevi muralidharan
SpeciesList	scientific name: Macadamia; NCBI TaxID: 4329;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ FT

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-09 03:21:52	ID requested
1	2020-01-13 04:44:03	announced
⏵ 2	2020-01-15 06:12:11	announced	2020-01-15: Updated publication reference for DOI(s): 10.1016/j.foodres.2019.108838.
3	2023-11-14 06:55:26	announced	2023-11-14: Updated project metadata.

10.1016/j.foodres.2019.108838;

submitter keyword: Macadamia nut

label-free shotgun proteomics

in silico analysis

allergenicity

cross-reactivity

N Alice Lee
contact affiliation	ARC Training Centre for Advanced Technologies in Food Manufacture, School of Chemical Engineering, UNSW SYDNEY, NSW 2052, AUSTRALIA
contact email	alice.lee@unsw.edu.au
lab head
sridevi muralidharan
contact affiliation	UNSW
contact email	sridevi03@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD015364

PRIDE project URI

• PRIDE
  1. PXD015364
     1. Label: PRIDE project
     2. Name: A label-free shotgun proteomics analysis of macadamia nut