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PXD015364-2

PXD015364 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA label-free shotgun proteomics analysis of macadamia nut
DescriptionIn this study, we present a first proteomic overview of macadamia nut using a label-free shotgun proteomic approach and prediction of their proteins with potential allergenic activity and cross-reactivity via an in silico analysis. The defense response proteins were the most abundant group of proteins and accounted for 24% of the total abundance. The vicilin-like macadamia antimicrobial peptides 2-3 (MiAMP2) was the most abundant protein. The cascading of amino acid and carbohydrate metabolic pathways in macadamia nut were constructed and proposed for the first time. The in silico analysis revealed seed storage proteins (conglutins and vicilins), rubber elongation factor proteins, phosphate binding proteins, and detoxifying methylglyoxalases among others as proteins with allergenic and cross-reactive potential. The label-free shotgun proteomics study revealed valuable insight into the genetic and biological makeup of macadamia nut and potential proteins with predictive allergenic activity and cross-reactivity. Integration of genomics with the proteomic data will be indispensable for future molecular characterization of macadamia including allergens.
HostingRepositoryPRIDE
AnnounceDate2020-01-13
AnnouncementXMLSubmission_2020-01-15_06:12:10.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmittersridevi muralidharan
SpeciesList scientific name: Macadamia; NCBI TaxID: 4329;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ FT
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-09-09 03:21:52ID requested
12020-01-13 04:44:03announced
22020-01-15 06:12:11announced2020-01-15: Updated publication reference for DOI(s): 10.1016/j.foodres.2019.108838.
32023-11-14 06:55:26announced2023-11-14: Updated project metadata.
Publication List
10.1016/j.foodres.2019.108838;
Keyword List
submitter keyword: Macadamia nut
label-free shotgun proteomics
in silico analysis
allergenicity
cross-reactivity
Contact List
N Alice Lee
contact affiliationARC Training Centre for Advanced Technologies in Food Manufacture, School of Chemical Engineering, UNSW SYDNEY, NSW 2052, AUSTRALIA
contact emailalice.lee@unsw.edu.au
lab head
sridevi muralidharan
contact affiliationUNSW
contact emailsridevi03@gmail.com
dataset submitter
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Dataset FTP location
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PRIDE project URI
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