PXD015359-2

PXD015359 is an original dataset announced via ProteomeXchange.

Title	Cell-surface proteomics identifies differences in signalling and adhesion protein expression between naive and primed human pluripotent stem cells
Description	Naive and primed human pluripotent stem cells (hPSC) provide valuable models to study cellular and molecular developmental processes. The lack of detailed information about cell-surface protein expression in these two pluripotent cell types prevents an understanding of how the cells communicate and interact with their microenvironments. Here, we used plasma membrane profiling to directly measure cell-surface protein expression in naive and primed hPSC. This unbiased approach quantified over 1700 plasma membrane proteins including those involved in cell adhesion, signalling and cell interactions. Notably, multiple cytokine receptors upstream of JAK-STAT signalling were more abundant in naive hPSC. In addition, functional experiments showed that FOLR1 and SUSD2 proteins are highly expressed at the cell surface in naive hPSC but are not required to establish human naive pluripotency. This study provides a comprehensive stem cell proteomic resource that uncovers differences in signalling pathway activity and has identified new markers to define human pluripotent states.
HostingRepository	PRIDE
AnnounceDate	2020-04-17
AnnouncementXML	Submission_2020-04-21_22:32:12.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Katarzyna Wojdyla
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-09 02:50:43	ID requested
1	2020-04-16 22:17:05	announced
⏵ 2	2020-04-21 22:32:13	announced	2020-04-22: Updated publication reference for PubMed record(s): 32302559.
3	2024-10-22 05:02:10	announced	2024-10-22: Updated project metadata.

Wojdyla K, Collier AJ, Fabian C, Nisi PS, Biggins L, Oxley D, Rugg-Gunn PJ, Cell-Surface Proteomics Identifies Differences in Signaling and Adhesion Protein Expression between Naive and Primed Human Pluripotent Stem Cells. Stem Cell Reports, 14(5):972-988(2020) [pubmed]

submitter keyword: plasma membrane profilling, naive and primed human pluripotent stem cells

Peter Rugg-Gunn
contact affiliation	Epigenetics Programme, The Babraham Institute, Cambridge, UK and Wellcome Trust - MRC Cambridge Stem Cell Institute, Cambridge, UK
contact email	peter.rugg-gunn@babraham.ac.uk
lab head
Katarzyna Wojdyla
contact affiliation	University of Cambridge
contact email	wojdyla.kat@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD015359

PRIDE project URI

• PRIDE
  1. PXD015359
     1. Label: PRIDE project
     2. Name: Cell-surface proteomics identifies differences in signalling and adhesion protein expression between naive and primed human pluripotent stem cells