PXD015331-3

PXD015331 is an original dataset announced via ProteomeXchange.

Title	Transcriptional regulation of ER-phagy by MiT/TFE factors controls chondrocyte differentiation and bone formation
Description	Lysosomal-autophagic degradation of Endoplasmic Reticulum via autophagy (ER-phagy) is emerging as critical regulator of ER homeostasis and function. However, the molecular mechanisms governing ER-phagy are still unknown. Working in chondrocytes, we found that ER-phagy and lysosome biogenesis are co-activated by FGF signaling during hypertrophic differentiation, a mandatory step for bone formation. FGF induced ER-phagy trough IRS1-dependent inhibition of the insulin signaling and activation of MiT/TFE transcription factors, master regulators of lysosome biogenesis. MiT/TFE promoted ER-phagy through the induction of the ER-phagy receptor FAM134B. Notably, the activation of ER-phagy promotes chondrocytes differentiation and secretion of factors required for cartilage replacement by bone. Consistently, medaka fish knock-down for FAM134B have impaired ossification of cranial bones. Thus, ER-phagy is a transcriptionally regulated process that participates to cell differentiation during development.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:09:02.848.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Natalie Krahmer
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-05 03:44:56	ID requested
1	2020-07-14 22:52:01	announced
2	2020-08-02 22:34:16	announced	2020-08-03: Updated publication reference for PubMed record(s): 32716134.
⏵ 3	2024-10-22 05:09:11	announced	2024-10-22: Updated project metadata.

10.15252/embj.2020105696;

Cinque L, De Leonibus C, Iavazzo M, Krahmer N, Intartaglia D, Salierno FG, De Cegli R, Di Malta C, Svelto M, Lanzara C, Maddaluno M, Wanderlingh LG, Huebner AK, Cesana M, Bonn F, Polishchuk E, H, ü, bner CA, Conte I, Dikic I, Mann M, Ballabio A, Sacco F, Grumati P, Settembre C, MiT/TFE factors control ER-phagy via transcriptional regulation of FAM134B. EMBO J, 39(17):e105696(2020) [pubmed]

curator keyword: Biological

submitter keyword: phosphoprotemics,FGF signaling, ER-phagy

Carmine Settembre
contact affiliation	Telethon Institute of Genetics and Medicine (TIGEM), Pozzuoli, Italy. Department of Medical and Translational Science, University of Naples "Federico II", Italy.
contact email	settembre@tigem.it
lab head
Natalie Krahmer
contact affiliation	Max Planck of Biochemistry
contact email	krahmer@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD015331

PRIDE project URI

• PRIDE
  1. PXD015331
     1. Label: PRIDE project
     2. Name: Transcriptional regulation of ER-phagy by MiT/TFE factors controls chondrocyte differentiation and bone formation