PXD015316-1

PXD015316 is an original dataset announced via ProteomeXchange.

Title	Proteomic Analysis of Measles Virus Protein C-Host Interactions in an Infectious Context Using Recombinant Viruses
Description	Viruses manipulate central machineries of host cells to their advantage. They prevent host cell antiviral responses in order to create a favourable environment for their survival and propagation. Measles virus (MV) encodes two non-structural proteins MV-V and MV-C, proposed to counteract the host interferon response and to regulate cell death pathways in various functional assays. Several molecular mechanisms underlining MV-V regulation of innate immunity and cell death responses have been proposed, whereas MV-C host protein partners are less studied. We suggest that some cellular factors that are controlled by MV-C protein during viral replication could be components of innate immunity and the cell death pathways. In order to determine which host factors are hijacked by MV-C, we captured both direct and indirect host protein partners of MV-C protein. For this we used a strategy based on recombinant viruses expressing tagged viral proteins followed by affinity purification and a bottom-up mass spectrometry analysis. A list of host proteins specifically interacting with MV-C protein in different cell lines was identified.
HostingRepository	PRIDE
AnnounceDate	2021-01-19
AnnouncementXML	Submission_2021-01-18_23:58:58.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thibaut Douché
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	L-methionine (S)-sulfoxide; N-acylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-04 06:11:45	ID requested
⏵ 1	2021-01-18 23:58:59	announced

Dataset with its publication pending

submitter keyword: Human, Infection, Measle virus, Interactomic, QExactive

Frédéric TANGY
contact affiliation	Viral Genomics and Vaccination Unit, Department of Virology, Institut Pasteur, CNRS UMR-3569, 75015 Paris, France
contact email	frederic.tangy@pasteur.fr
lab head
Thibaut Douché
contact affiliation	Institut Pasteur
contact email	thibaut.douche@pasteur.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD015316

PRIDE project URI

• PRIDE
  1. PXD015316
     1. Label: PRIDE project
     2. Name: Proteomic Analysis of Measles Virus Protein C-Host Interactions in an Infectious Context Using Recombinant Viruses