PXD015267-1

PXD015267 is an original dataset announced via ProteomeXchange.

Title	Quantitative and phosphorylated proteomics data on the dynamic changes of ovarian developmental proteins in female haemaphysalis longicornis
Description	Background: Haemaphysalis longicornis is an obligate blood-feeding ectoparasite that can spread a variety of pathogens, causing serious damage to humans, livestock, and wildlife. With extremely high reproductive capability, female ticks can ingest a large amount of blood from their hosts, causing extreme harm. The degree of ovarian development of females directly affects the number of eggs laid by female ticks. Studying the regulatory mechanism of tick ovary development is of great significance for effectively controlling tick populations and thus reducing tick damage. Methods: In this study, quantitative proteomics based on data independent acquisition (DIA) was used to systematically study dynamic changes in the expression levels of all proteins and in protein phosphorylation during the ovarian development of engorged females. Results: In ovary development, the synergistic action of many proteins is required to rapidly achieve ovarian development and egg formation. Leucine aminopeptidase, acting as a hydrolase, provides amino acids for ovarian development by hydrolysing proteins or peptides. When it was knocked down by RNAi, ovarian development was inhibited. Conclusions: The results of this study found many proteins that play important role during ovarian development, and also reveal the importance of protein phosphorylation in ovarian development, which is an important reproductive strategy in which ticks can rapidly and efficiently convert large amounts of blood in the ovaries into egg-producing raw materials and ultimately produce many eggs.
HostingRepository	iProX
AnnounceDate	2019-09-01
AnnouncementXML	Submission_2019-09-01_19:30:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hui Wang
SpeciesList	scientific name: Haemaphysalis longicornis; NCBI TaxID: 44386;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-01 19:26:24	ID requested
⏵ 1	2019-09-01 19:30:09	announced

Dataset with its publication pending

submitter keyword: Haemaphysalis longicornis, Ovary, Proteomics, DIA, RNAi

Hui Wang
contact affiliation	Hebei normal university
contact email	whui1981@163.com
lab head
Hui Wang
contact affiliation	Hebei normal university
contact email	whui1981@163.com
dataset submitter

iProX dataset URI