PXD015262-1

PXD015262 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	ClpX, activated by SSB, is required for DNA replication in Mycobacterium tuberculosis
Description	The ClpP1P2 proteolytic complex is essential in Mycobacterium tuberculosis (Mtb). ClpP1P2 mediated proteolysis by ClpP1P2 requires an associated ATPase, either ClpX or ClpC1. Here, we seek to define the unique contributions of the ClpX ATPase to mycobacterial growth. We formally demonstrate that ClpX is essential for mycobacterial growth and to understand its essential functions, we identify ClpX-His-interacting proteins by pulldown and tandem mass spectrometry. We find an unexpected association between ClpX and proteins involved in DNA replication, and confirm a physical association between ClpX and the essential DNA maintenance protein Single-Stranded DNA Binding protein (SSB). Although pure SSB is not degraded by ClpXP1P2, it instead enhances ATPase hydrolysis by ClpX and degradation of the model substrate GFP-SsrA by ClpXP1P2. This activation of ClpX is mediated by the C-terminal tail of SSB that had previously been implicated in the activation of other ATPases associated with DNA replication. Consistent with the predicted interactions, depletion of ClpX perturbs DNA replication. These data reveal that ClpX serves a necessary function in DNA replication and identify the first activator of ClpX in mycobacteria.
HostingRepository	MassIVE
AnnounceDate	2019-08-31
AnnouncementXML	Submission_2019-08-31_08:25:16.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jemila Kester
SpeciesList	scientific name: Mycobacterium smegmatis; NCBI TaxID: 1772;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-31 08:15:48	ID requested
⏵ 1	2019-08-31 08:25:16	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: ClpX, ATPase, ATPgammaS, substrate

submitter keyword: ClpX, ATPase, ATPgammaS, substrate

Contact List

Sarah Fortune
contact affiliation	HSPH
contact email	sfortune@hsph.harvard.edu
lab head
Jemila Kester
contact affiliation	HSPH
contact email	jemilacaplan@gmail.com
lab head
Jemila Kester
contact affiliation	HSPH
contact email	jemilacaplan@gmail.com
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000084267/