PXD015258-1
PXD015258 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | TMT10 controlled mixtures - SILAC HeLa UPS1, acquired using SPS-MS3 |
| Description | The controlled mixtures were prepared to evaluate the ability of our proposed approach when dealing with the complicated design, e.g. multiple TMT mixtures with technical replicates. 500, 333, 250, and 62.5 fmol UPS1 peptides were spiked-into 50 g SILAC HeLa peptides in duplicate. It produced a dilution series corresponding to 1, 0.667, 0.5, and 0.125 of the highest UPS1 peptide amount (500 fmol). In addition, a reference sample was generated by pooling all four diluted UPS1 peptide samples (286.5 fmol) and combined with 50 g of SILAC HeLa in duplicate. These 10 replicates were labeled with TMT10-plex reagents and mixed together to pass LC-MS/MS analysis. The procedure was repeated to generate a total of five such controlled mixtures. To assess technical variability, three technical replicates were prepared for each mixture. Totally there are 15 MS runs from 5 TMT mixtures. LC-MS/MS was performed using an EASY-nLC 1200 ultrahigh pressure liquid chromatography (UHPLC) connected to an Orbitrap Fusion Lumos Tribrid and equipped with an EASY-spray source (Thermo Fisher Scientific, San Jose, CA). The data was acquired using an MS2/MS3 (also called multinotch MS3 or Synchronous Precursor Selection, SPS). |
| HostingRepository | MassIVE |
| AnnounceDate | 2019-09-03 |
| AnnouncementXML | Submission_2019-09-03_08:18:21.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Meena Choi |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2019-08-30 15:46:21 | ID requested | |
| ⏵ 1 | 2019-09-03 08:18:21 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: TMT10plex, Controlled Mixture, Lumos, Proteomics |
Contact List
| Tom Dunkley | |
|---|---|
| contact affiliation | Roche Pharma Research and Early Development, Pharmaceutical Sciences, Roche Innovation Center Basel, Hoffmann-La Roche Ltd |
| contact email | tom.dunkley@roche.com |
| lab head | |
| Olga Vitek | |
| contact affiliation | Khoury College of Computer Sciences, Northeastern University |
| contact email | o.vitek@northeastern.edu |
| lab head | |
| Meena Choi | |
| contact affiliation | Northeastern University |
| contact email | mnchoi67@gmail.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000084264/ |
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