PXD015205-1

PXD015205 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Determinants of site-specific dephosphorylation by PP2A-B56
Description	The tumor suppressor PP2A is a major cellular protein phosphatase that regulates numerous cellular processes through the formation of holoenzymes containing distinct regulatory B-subunits. However, the determinants of differential substrate dephosphorylation by PP2A are poorly understood. Here, we develop a specific genetically encoded inhibitor of PP2A-B56 and perform global phosphoproteomic studies to identify hundreds of regulated phosphorylation sites. We show that PP2A-B56 substrate specificity is controlled by affinity and position of B56 binding motifs and that PP2A active site preference is determined by the B-subunit. These insights uncover PP2A-B56 as a novel negative regulator of ADAM17 mediated growth factor signalling and tumor development. Collectively our work identifies basic principles of PP2A-B56 specificity with broad implications for understanding signalling in eukaryotes.
HostingRepository	MassIVE
AnnounceDate	2020-05-04
AnnouncementXML	Submission_2020-05-04_06:49:07.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Mark Adamo
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	TMT6plex; Phospho; Oxidation; Carbamidomethyl
Instrument	instrument model; Orbitrap Fusion; instrument model

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-28 14:13:19	ID requested
⏵ 1	2020-05-04 06:49:08	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: PP2A, B56, phosphatase, holoenzyme, dephosphorylation, motif, ADAM17, growth factor, tumor

submitter keyword: PP2A, B56, phosphatase, holoenzyme, dephosphorylation, motif, ADAM17, growth factor, tumor

Contact List

Arminja Kettenbach
contact affiliation	The Geisel School of Medicine at Dartmouth
contact email	arminja.n.kettenbach@dartmouth.edu
lab head
Mark Adamo
contact affiliation	Dartmouth College
contact email	mark.e.adamo@dartmouth.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000084245/