PXD015194 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative proteomics of Uukuniemi virus - host cell interactions reveals GBF1 as proviral host factor for phleboviruses |
Description | Novel tick-borne phleboviruses in the Phenuiviridae family, which are highly pathogenic in humans and all closely related to Uukuniemi virus (UUKV), have recently emerged on different continents. How phleboviruses assemble, bud, and exit cells remains largely elusive. Here, we performed high-resolution, label-free mass spectrometry analysis of UUKV immuno-precipitated from cell lysates and identified 39 cellular partners interacting with the viral envelope glycoproteins. Hit validation by a subsequent RNA interference screen revealed Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 (GBF1), a guanine nucleotide exchange factor resident in the Golgi, as a critical host factor required for the UUKV life cycle. An inhibitor of GBF1, Golgicide A, confirmed the role of the cellular factor in UUKV infection. We could pinpoint the GBF1 requirement to UUKV replication and particle assembly. When the investigation was extended to viruses from various positive and negative RNA viral families, we found that not only phleboviruses rely on GBF1 for infection, but also Flavi-, Corona-, Rhabdo-, and Togaviridae. In contrast, silencing or blocking GBF1 did not abrogate infection by the human adenovirus serotype 5 and immunodeficiency retrovirus type 1, the replication of both occurs in the nucleus. Together our results indicate that UUKV relies on GBF1 for viral replication, assembly and egress. This study also highlights the proviral activity of GBF1 in the infection by a broad range of important zoonotic RNA viruses. |
HostingRepository | PRIDE |
AnnounceDate | 2019-10-02 |
AnnouncementXML | Submission_2019-10-02_01:32:51.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Mario Oroshi |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-08-28 01:18:01 | ID requested | |
⏵ 1 | 2019-10-02 01:32:52 | announced | |
2 | 2019-11-08 00:39:24 | announced | 2019-11-08: Updated publication reference for PubMed record(s): 31570497. |
3 | 2019-11-27 06:01:22 | announced | 2019-11-27: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Uukuniemi, Phenuiviridae, phlebovirus, viral glycoprotein interactions, quantitative proteomics, GBF1, alphavirus, flavivirus, hepacivirus, rhabdovirus, coronavirus, adenovirus, retrovirus, viral replication, viral assembly, viral egress |
Contact List
Felix Meissner |
contact affiliation | Experimental Systems Immunology, Max Planck Institute of Biochemistry |
contact email | meissner@biochem.mpg.de |
lab head | |
Mario Oroshi |
contact affiliation | Proteomics |
contact email | oroshi@biochem.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD015194
- Label: PRIDE project
- Name: Quantitative proteomics of Uukuniemi virus - host cell interactions reveals GBF1 as proviral host factor for phleboviruses