PXD015167-2

PXD015167 is an original dataset announced via ProteomeXchange.

Title	Global proteomic analysis for stress response of Mycobacterium tuberculosis
Description	To expand an insight into the stress adaptation in Mycobacterium tuberculosis H37Rv (Mtb), we approached the iTRAQ analysis to understand the global proteome profile of stressed Mtb under Acid (5.5 pH), Diamide (5mM) and Hydrogen peroxide (H2O2) (5mM) conditions and compared with gene expression of Mtb incubated in 7H9 rich medium (pH 7.0). The experiment was performed for overnight treatment of aforementioned stress. Whole cell lysate (WCL) from three independent repeats of control and stressed samples were labelled with three set of 4-plex iTRAQ labelling reagents (AB SCIEX) in proportion to manufacturer’s protocol.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:33:33.108.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nisheeth Agarwal
SpeciesList	scientific name: Mycobacterium tuberculosis; NCBI TaxID: NCBITaxon:1773; scientific name: Mycobacterium tuberculosis H37Rv; NCBI TaxID: 83332;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-08-27 05:51:35	ID requested
1	2022-10-13 05:22:22	announced
⏵ 2	2023-11-14 07:33:34	announced	2023-11-14: Updated project metadata.

Choudhary E, Sharma R, Pal P, Agarwal N, in Response to Acid and Oxidative Stresses. ACS Omega, 7(30):26749-26766(2022) [pubmed]

submitter keyword: iTRAQ MTB stres response

Nisheeth Agarwal
contact affiliation	Translational Helath Science and Technology Institute, NCR Biotech Science Cluster, Faridabad , Haryana, India ( lab head )
contact email	nisheeth@thsti.res.in
lab head
Nisheeth Agarwal
contact affiliation	Professor
contact email	nishu1001@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD015167

PRIDE project URI

• PRIDE
  1. PXD015167
     1. Label: PRIDE project
     2. Name: Global proteomic analysis for stress response of Mycobacterium tuberculosis